ArticlePloS one2013
The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 38 citations in OpenAlex.
- Phylogenetic distribution and longitudinal persistence of plasmids inMicrobial genomics · 2026Article
- Widespread macrolide resistance among rapidly growing mycobacteria due to plasmids containing erm(55).Scientific reports · 2026Article
- Genomic insights into the plasmidome of non-tuberculous mycobacteria.Genome medicine · 2025Article
- Plasmid-mediated drug resistance in mycobacteria: the tip of the iceberg?Journal of clinical microbiology · 2023Article
- Emergence of Inducible Macrolide Resistance in Mycobacterium chelonae Due to Broad-Host-Range Plasmid and Chromosomal Variants of the Novel 23S rRNA Methylase Gene,Journal of clinical microbiology · 2023Article
- Genome reorganization during emergence of host-associatedMicrobial genomics · 2021Article
- Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense.Scientific reports · 2021Article
- Pipeline of anti-Mycobacterium abscessus small molecules: Repurposable drugs and promising novel chemical entities.Medicinal research reviews · 2021Review
- ComprehensiveMicrobial genomics · 2021Article
- Revealing biophysical properties of KfrA-type proteins as a novel class of cytoskeletal, coiled-coil plasmid-encoded proteins.BMC microbiology · 2021Article
- Non-Tuberculous Mycobacteria: Molecular and Physiological Bases of Virulence and Adaptation to Ecological Niches.Microorganisms · 2020Review
- Genomics of Atlantic ForestMicrobial genomics · 2020Article
- Comparative Analysis of Whole-Genome and Methylome Profiles of a Smooth and a RoughG3 (Bethesda, Md.) · 2020Article
- Where the plasmids roam: large-scale sequence analysis reveals plasmids with large host ranges.Microbial genomics · 2019Article
- A Closer Look at the Genomic Variation of Geographically DiverseFrontiers in microbiology · 2018Review
- Genomic epidemiology of a national outbreak of post-surgicalMicrobial genomics · 2017Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Mycobacterial Pan-Genome Analysis Suggests Important Role of Plasmids in the Radiation of Type VII Secretion Systems.Genome biology and evolution · 2016Article
- fosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin.Antimicrobial agents and chemotherapy · 2016Article
- Two types of genetic carrier, the IncP genomic island and the novel IncP-1β plasmid, for the aac(2')-IIa gene that confers kasugamycin resistance in Acidovorax avenae ssp. avenae.Molecular plant pathology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAn extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004-2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a ∼50 kb band. The nature of this band was investigated. METHODOLOGY/PRINCIPAL
findingsGenomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,267-bp [corrected] circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1β and is highly related to BRA100, pJP4, pAKD33 and pB10. The presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. The plasmid was visualized as a ∼50-kb band using PFGE and Southern blot hybridization in 12 isolates. The remaining 25 isolates did not exhibit any evidence of this plasmid. The plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc(2)155 could not be demonstrated. CONCLUSIONS/SIGNIFICANCE: The occurrence of a broad-host-range IncP-1β plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.
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