ArticlePloS one2013
An efficient method for dorsal root ganglia neurons purification with a one-time anti-mitotic reagent treatment.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Primary Cell Cultures in Neurobiology: Optimized Protocol for Culture of Mouse Fetal Hindbrain Neurons.Cells · 2025Article
- Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair.Bioengineering & translational medicine · 2024Article
- Human sensory-like neuron cultivation-An optimized protocol.Frontiers in neuroscience · 2024Article
- Synapses without tension fail to fire in an in vitro network of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Article
- Astrocyte-mediated Transduction of Muscle Fiber Contractions Synchronizes Hippocampal Neuronal Network Development.Neuroscience · 2023Article
- Dorsal root ganglion neurons recapitulate the traumatic axonal injury of CNS neurons in response to a rapid stretchFrontiers in cellular neuroscience · 2023Article
- Implantable Electroceutical Approach Improves Myelination by Restoring Membrane Integrity in a Mouse Model of Peripheral Demyelinating Neuropathy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Raf kinase inhibitory protein reduces bradykinin receptor desensitization.Journal of neurochemistry · 2022Article
- Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons.Iranian journal of basic medical sciences · 2021Article
- Usefulness of the measurement of neurite outgrowth of primary sensory neurons to study cancer-related painful complications.Biochemical pharmacology · 2021Article
- WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury.International journal of molecular sciences · 2020Article
- A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals.Journal of visualized experiments : JoVE · 2020Article
- 3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response.ACS applied materials & interfaces · 2017Article
- Identification of a signaling cascade that maintains constitutive δ-opioid receptor incompetence in peripheral sensory neurons.The Journal of biological chemistry · 2017Article
- Pannexin 1 Modulates Axonal Growth in Mouse Peripheral Nerves.Frontiers in cellular neuroscience · 2017Article
- Development of a spontaneously active dorsal root ganglia assay using multiwell multielectrode arrays.Journal of neurophysiology · 2016Article
- A cortical astrocyte subpopulation inhibits axon growth in vitro and in vivo.Molecular medicine reports · 2015Article
- Dorsal root ganglia neurons and differentiated adipose-derived stem cells: an in vitro co-culture model to study peripheral nerve regeneration.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe dorsal root ganglia (DRG) neuron is an invaluable tool in axon growth, growth factor regulation, myelin formation and myelin-relevant researches. The purification of DRG neurons is a key step in these studies. Traditionally, purified DRG neurons were obtained in two weeks after exposure to several rounds of anti-mitotic reagent. METHODS AND
resultsIn this report, a novel, simple and efficient method for DRG purification is presented. DRG cultures were treated once with a high-dose anti-mitotic reagent cocktail for 72 hours. Using this new method, DRG neurons were obtained with 99% purification within 1 week. We confirmed that the neurite growth and the viability of the purified DRG neurons have no difference from the DRG neurons purified by traditional method. Furthermore, P0 and MBP expression was observed in myelin by immunocytochemistry in the DRG/SC co-culture system. The formation of mature node of Ranvier in DRG-Schwann cell co-culture system was observed using anti-Nav 1.6 and anti-caspr antibody. CONCLUSION AND SIGNIFICANCE: The results indicate that this high dose single treatment did not compromise the capacity of DRG neurons for myelin formation in the DRG/SC co-culture system. In conclusion, a convenient approach for purifying DRG neurons was developed which is time-saving and high-efficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.