ArticlePloS one2013
Application of an integrated statistical design for optimization of culture condition for ammonium removal by Nitrosomonas europaea.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Bioprocess Optimization of Nutritional Parameters for Enhanced Anti-leukemic L-Asparaginase Production byInternational journal of peptide research and therapeutics · 2021Article
- Optimization of Cultural Conditions forBioMed research international · 2018Article
- High cell density cultivation of the chemolithoautotrophic bacterium Nitrosomonas europaea.Folia microbiologica · 2016Article
- Enhanced textile dye decolorization by marine-derived basidiomycete Peniophora sp. CBMAI 1063 using integrated statistical design.Environmental science and pollution research international · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Statistical methodology was applied to the optimization of the ammonium oxidation by Nitrosomonas europaea for biomass concentration (C(B)), nitrite yield (Y(N)) and ammonium removal (R(A)). Initial screening by Plackett-Burman design was performed to select major variables out of nineteen factors, among which NH4Cl concentration (C(N)), trace element solution (TES), agitation speed (AS), and fermentation time (T) were found to have significant effects. Path of steepest ascent and response surface methodology was applied to optimize the levels of the selected factors. Finally, multi-objective optimization was used to obtain optimal condition by compromise of the three desirable objectives through a combination of weighted coefficient method coupled with entropy measurement methodology. These models enabled us to identify the optimum operation conditions (C(N)= 84.1 mM; TES = 0.74 ml; AS= 100 rpm and T = 78 h), under which C(B)= 3.386×10(8) cells/ml; Y(N)= 1.98 mg/mg and R(A) = 97.76% were simultaneously obtained. The optimized conditions were shown to be feasible through verification tests.
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