ArticlePloS one2013
Molecular identification of falciparum malaria and human tuberculosis co-infections in mummies from the Fayum depression (Lower Egypt).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 87 citations in OpenAlex.
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- Intra-individual variability in ancient plasmodium DNA recovery highlights need for enhanced sampling.Scientific reports · 2025Article
- The World Health Organization's declaration of Egypt as malaria free will boost malaria control efforts in the region.New microbes and new infections · 2024Article
- The Egypt Genome Project.Nature genetics · 2024Article
- Use of rapid diagnostic tests for the detection of ancient malaria infections in dental pulp from the sixth century in Versailles, France.Malaria journal · 2023Article
- Malaria and tuberculosis co-infection-a review.Oxford open immunology · 2023Review
- Preventive Measures against Pandemics from the Beginning of Civilization to Nowadays-How Everything Has Remained the Same over the Millennia.Journal of clinical medicine · 2022Review
- The elusive parasite: comparing macroscopic, immunological, and genomic approaches to identifying malaria in human skeletal remains from Sayala, Egypt (third to sixth centuries AD).Archaeological and anthropological sciences · 2021Article
- Malaria in Europe: A Historical Perspective.Frontiers in medicine · 2021Review
- 2000-year-old pathogen genomes reconstructed from metagenomic analysis of Egyptian mummified individuals.BMC biology · 2020Article
- The paleopathological evidence on the origins of human tuberculosis: a review.Journal of preventive medicine and hygiene · 2020Review
- Assessing the Parasitic Burden in a Late Antique Florentine Emergency Burial Site.The Korean journal of parasitology · 2019Article
- Prevalence of Malaria and TB Coinfection at a National Tuberculosis Treatment Centre in Uganda.Journal of tropical medicine · 2019Article
- The Immunodetection of Non-Falciparum Malaria in Ancient Egyptian Bones (Giza Necropolis).BioMed research international · 2018Article
- Paleopathological Considerations on Malaria Infection in Korea before the 20th Century.BioMed research international · 2018Review
- Ancient Egyptian mummy genomes suggest an increase of Sub-Saharan African ancestry in post-Roman periods.Nature communications · 2017Article
- Molecular Screening for Malaria among Blood Donors in a WHO Claimed Region of Egypt, Fayoum Governorate.Mediterranean journal of hematology and infectious diseases · 2017Article
- Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- The threat of antimalarial drug resistance.Tropical diseases, travel medicine and vaccines · 2016Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the presence of the lake Quarun and to the particular nature of its irrigation system, it has been speculated that the Fayum, a large depression 80 kilometers south-west of modern Cairo, was exposed to the hazards of malaria in historic times. Similarly, it has been speculated that, in the same area, also human tuberculosis might have been far more widespread in the antiquity than in its recent past. If these hypotheses were confirmed, it would imply that frequent cases of co-infection between the two pathogens might have occurred in ancient populations. To substantiate those speculations, molecular analyses were carried out on sixteen mummified heads recovered from the necropolis of Abusir el Meleq (Fayum) dating from the 3(rd) Intermediate Period (1064-656 BC) to the Roman Period (30 BC-300 AD). Soft tissue biopsies were used for DNA extractions and PCR amplifications using well-suited protocols. A partial 196-bp fragment of Plasmodium falciparum apical membrane antigen 1 gene and a 123-bp fragment of the Mycobacterium tuberculosis complex insertion sequence IS6110 were amplified and sequenced in six and five of the sixteen specimens, respectively. A 100% concordance rates between our sequences and those of P. falciparum and M. tuberculosis complex ones were obtained. Lastly, concomitant PCR amplification of P. falciparum and M. tuberculosis complex DNA specific fragments was obtained in four mummies, three of which are (14)C dated to the Late and Graeco-Roman Periods. Our data confirm that the hydrography of Fayum was extremely conducive to the spread of malaria. They also support the notion that the agricultural boom and dense crowding occurred in this region, especially under the Ptolemies, highly increased the probability for the manifestation and spread of tuberculosis. Here we extend back-wards to ca. 800 BC new evidence for malaria tropica and human tuberculosis co-occurrence in ancient Lower Egypt.
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