ArticlePloS one2013
Combined fluorescent-chromogenic in situ hybridization for identification and laser microdissection of interphase chromosomes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Blank spots on the map: some current questions on nuclear organization and genome architecture.Histochemistry and cell biology · 2018Review
- Chromosome positioning and male infertility: it comes with the territory.Journal of assisted reproduction and genetics · 2018Review
- Spatial organization of chromosome territories in the interphase nucleus of trisomy 21 cells.Chromosoma · 2018Article
- A Pair of Maternal Chromosomes Derived from Meiotic Nondisjunction in Trisomy 21 Affects Nuclear Architecture and Transcriptional Regulation.Scientific reports · 2017Article
- Chromosomal aneuploidies induced upon Lamin B2 depletion are mislocalized in the interphase nucleus.Chromosoma · 2017Article
- Spatial Genome Organization and Its Emerging Role as a Potential Diagnosis Tool.Frontiers in genetics · 2016Review
- The Effects of Disease Models of Nuclear Actin Polymerization on the Nucleus.Frontiers in physiology · 2016Article
- Expression of the DYRK1A gene correlates with its 3D positioning in the interphase nucleus of Down syndrome cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome territories constitute the most conspicuous feature of nuclear architecture, and they exhibit non-random distribution patterns in the interphase nucleus. We observed that in cell nuclei from humans with Down Syndrome two chromosomes 21 frequently localize proximal to one another and distant from the third chromosome. To systematically investigate whether the proximally positioned chromosomes were always the same in all cells, we developed an approach consisting of sequential FISH and CISH combined with laser-microdissection of chromosomes from the interphase nucleus and followed by subsequent chromosome identification by microsatellite allele genotyping. This approach identified proximally positioned chromosomes from cultured cells, and the analysis showed that the identity of the chromosomes proximally positioned varies. However, the data suggest that there may be a tendency of the same chromosomes to be positioned close to each other in the interphase nucleus of trisomic cells. The protocol described here represents a powerful new method for genome analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.