ArticleFrontiers in microbiology2013
Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 81 citations in OpenAlex.
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- Impact of Copper on the Physiology and Transcriptome of Methylosinus Trichosporium OB3b Grown on Either Methane or Methanol.Environmental microbiology · 2026Article
- Methylocystis borbori sp.nov., a novel methanotrophic bacterium from the sludge of a freshwater lake and its metabolic properties.Antonie van Leeuwenhoek · 2024Article
- Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox.Synthetic and systems biotechnology · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- Resilience of aerobic methanotrophs in soils; spotlight on the methane sink under agriculture.FEMS microbiology ecology · 2024Article
- Methane biohydroxylation into methanol byFrontiers in bioengineering and biotechnology · 2024Article
- Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds.Advances in biochemical engineering/biotechnology · 2022Article
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath.Applied and environmental microbiology · 2021Article
- Biomedical Applications of Biomolecules Isolated from Methanotrophic Bacteria in Wastewater Treatment Systems.Biomolecules · 2021Review
- Transcriptomic and Metabolomic Responses to Carbon and Nitrogen Sources in Methylomicrobium album BG8.Applied and environmental microbiology · 2021Article
- Genome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1.World journal of microbiology & biotechnology · 2021Article
- Genome-Scale Metabolic Model Reconstruction and in Silico Investigations of Methane Metabolism inMicroorganisms · 2020Article
- Quantifying Methane and Methanol Metabolism of "mSystems · 2019Article
- Unusual and Highly Bioactive Sesterterpenes Synthesized byApplied and environmental microbiology · 2019Article
- Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor.Microbial cell factories · 2018Article
- Rare Earth Elements Alter Redox Balance inFrontiers in microbiology · 2018Article
- The Completed PacBio Single-Molecule Real-Time Sequence ofGenome announcements · 2017Article
- Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route.Microbial cell factories · 2017Article
- Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work we use metabolomics and (13)C-labeling data to refine central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotrophic bacterium. We demonstrate here that similar to non-methane utilizing methylotrophic alphaproteobacteria the core metabolism of the microbe is represented by several tightly connected metabolic cycles, such as the serine pathway, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle. Both in silico estimations and stable isotope labeling experiments combined with single cell (NanoSIMS) and bulk biomass analyses indicate that a significantly larger portion of the cell carbon (over 60%) is derived from CO2 in this methanotroph. Our(13) C-labeling studies revealed an unusual topology of the assimilatory network in which phosph(enol) pyruvate/pyruvate interconversions are key metabolic switches. A set of additional pathways for carbon fixation are identified and discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.