ArticleFrontiers in physiology2013
Close Association of Carbonic Anhydrase (CA2a and CA15a), Na(+)/H(+) Exchanger (Nhe3b), and Ammonia Transporter Rhcg1 in Zebrafish Ionocytes Responsible for Na(+) Uptake.
Article in Frontiers in physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Analysis of Genetic Diversity in Speckled Blue Grouper (Animals : an open access journal from MDPI · 2026Article
- Intracellular acid-base regulation mediates a trade-off between shell and somatic growth in a clam under ocean acidification.iScience · 2026Article
- Population Genomics Provides Novel Insights Into Evolutionary Relationships and Local Adaptation of Two EcotypesEcology and evolution · 2025Article
- Making sense of carbonic anhydrase function in zebrafish using antisense morpholinos.Molecular genetics and genomics : MGG · 2025Review
- Review
- Role of the kidneys in acid-base regulation and ammonia excretion in freshwater and seawater fish: implications for nephrocalcinosis.Frontiers in physiology · 2023Review
- Roles of Carbonic Anhydrases and Carbonic Anhydrase Related Proteins in Zebrafish.International journal of molecular sciences · 2022Review
- Mechanisms of NaFrontiers in physiology · 2022Review
- Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine.Science advances · 2021Article
- Carbonic anhydrase inhibitor induces otic hair cell apoptosis via an intrinsic pathway and ER stress in zebrafish larvae.Toxicology reports · 2021Article
- Expression of ion transport genes in ionocytes isolated from larval zebrafish (American journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Xenobiotic transporter activity in zebrafish embryo ionocytes.Aquatic toxicology (Amsterdam, Netherlands) · 2019Article
- Physiological protective action of dissolved organic carbon on ion regulation and nitrogenous waste excretion of zebrafish (Danio rerio) exposed to low pH in ion-poor water.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- The physiology of the Tambaqui (Colossoma macropomum) at pH 8.0.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis.International journal of molecular sciences · 2018Review
- Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear.Marine biotechnology (New York, N.Y.) · 2017Article
- A Carbonic Anhydrase Serves as an Important Acid-Base Regulator in Pacific Oyster Crassostrea gigas Exposed to Elevated COMarine biotechnology (New York, N.Y.) · 2017Article
- NaFrontiers in physiology · 2017Article
- Article
- A broader look at ammonia production, excretion, and transport in fish: a review of impacts of feeding and the environment.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freshwater (FW) fishes actively absorb salt from their environment to tolerate low salinities. We previously reported that vacuolar-type H(+)-ATPase/mitochondrion-rich cells (H-MRCs) on the skin epithelium of zebrafish larvae (Danio rerio) are primary sites for Na(+) uptake. In this study, in an attempt to clarify the mechanism for the Na(+) uptake, we performed a systematic analysis of gene expression patterns of zebrafish carbonic anhydrase (CA) isoforms and found that, of 12 CA isoforms, CA2a and CA15a are highly expressed in H-MRCs at larval stages. The ca2a and ca15a mRNA expression were salinity-dependent; they were upregulated in 0.03 mM Na(+) water whereas ca15a but not ca2a was down-regulated in 70 mM Na(+) water. Immunohistochemistry demonstrated cytoplasmic distribution of CA2a and apical membrane localization of CA15a. Furthermore, cell surface immunofluorescence staining revealed external surface localization of CA15a. Depletion of either CA2a or CA15a expression by Morpholino antisense oligonucleotides resulted in a significant decrease in Na(+) accumulation in H-MRCs. An in situ proximity ligation assay demonstrated a very close association of CA2a, CA15a, Na(+)/H(+) exchanger 3b (Nhe3b), and Rhcg1 ammonia transporter in H-MRC. Our findings suggest that CA2a, CA15a, and Rhcg1 play a key role in Na(+)uptake under FW conditions by forming a transport metabolon with Nhe3b.
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