ArticleGenetica2013
Germline transformation of the spotted wing drosophilid, Drosophila suzukii, with a piggyBac transposon vector.
Article in Genetica, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Establishment of transgenic Drosophila suzukii lines that express phiC31 integrase and carry the sepia gene as a marker for transformation.Insect molecular biology · 2025Article
- Validation of heat-inducibleiScience · 2024Article
- Arthropod promoters for genetic control of disease vectors.Trends in parasitology · 2024Review
- Validation of a heat-induciblebioRxiv : the preprint server for biology · 2023Article
- ImprovedInternational journal of molecular sciences · 2023Article
- Sequence and expression analysis of the spermatogenesis-specific gene cognates, wampa and Prosα6T, in Drosophila suzukii.Genetica · 2023Article
- A transgenic female killing system for the genetic control of Drosophila suzukii.Scientific reports · 2021Article
- Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.BMC genetics · 2020Article
- Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.BMC genetics · 2020Article
- A Cross-Sectional Survey of Biosafety Professionals Regarding Genetically Modified Insects.Applied biosafety : journal of the American Biological Safety Association · 2020Article
- Identification and functional analysis of promoters of heat-shock genes from the fall armyworm, Spodoptera frugiperda.Scientific reports · 2020Article
- Synthetically engineeredProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Reduced Drosophila suzukii Infestation in Berries Using Deterrent Compounds and Laminate Polymer Flakes.Insects · 2017Article
- Temperature-dependent sex-reversal by a transformer-2 gene-edited mutation in the spotted wing drosophila, Drosophila suzukii.Scientific reports · 2017Article
- Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti.Scientific reports · 2017Article
- Insect transformation with piggyBac: getting the number of injections just right.Insect molecular biology · 2016Article
- A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila suzukii is a pest of small fruits in many parts of the world, whose management is limited to cultural practices and the use of insecticides. Here we describe a method to genetically manipulate this species in the first step to create female lethality strains useful for the sterile insect technique method of population suppression. This was achieved by the germ-line transformation of D. suzukii with a piggyBac transposon vector having a female-specific lethality effector construct. This can be used in a tetracycline-suppressible conditional gene expression system, when crossed to a suitable tet-transactivator strain. Transformation occurred efficiently, at a frequency of 16 % per fertile G0 embryo injected with vector and helper transposase plasmids. The vector was marked for transformant selection with the polyubiquitin-regulated EGFP fluorescent protein, and contains the attP landing site and heterospecific lox recombination sites for post-integration modification of the transgene vector. The 3xP3-AmCyan fluorescent protein marker was inserted within the lox sites to follow a possible recombinase-mediated cassette exchange, that would allow subsequent improvement of the transgenic strain by immobilization of the vector and introduction of new marker cassettes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.