ArticleMolecular and cellular biochemistry2013
Pulsed electric current induces the differentiation of human keratinocytes.
Article in Molecular and cellular biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Making Sense of Electrical Stimulation: A Meta-analysis for Wound Healing.Annals of biomedical engineering · 2024Pooled it
- A Data Driven Review of In Vitro Electrical and Mechanical Stimulation for Post-Acute Phase Wound Healing.Advanced healthcare materials · 2026Review
- Electroactive Electrospun Nanofibrous Scaffolds: Innovative Approaches for Improved Skin Wound Healing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Pulsed electrical stimulation and amino acid derivatives promote collagen gene expression in human dermal fibroblasts.Cytotechnology · 2024Article
- Possible Synergies of Nanomaterial-Assisted Tissue Regeneration in Plasma Medicine: Mechanisms and Safety Concerns.Nanomaterials (Basel, Switzerland) · 2022Review
- Effects of pulsed electrical stimulation on growth factor gene expression and proliferation in human dermal fibroblasts.Molecular and cellular biochemistry · 2021Article
- Repeated short climatic change affects the epidermal differentiation program and leads to matrix remodeling in a human organotypic skin model.Clinical, cosmetic and investigational dermatology · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although normal human keratinocytes are known to migrate toward the cathode in a direct current (DC) electric field, other effects of the electric stimulation on keratinocyte activities are still unclear. We have investigated the keratinocyte differentiation under monodirectional pulsed electric stimulation which reduces the electrothermal and electrochemical hazards of a DC application. When cultured keratinocytes were exposed to the electric field of 3 V (ca. 100 mV/mm) or 5 V (ca. 166 mV/mm) at a frequency of 4,800 Hz for 5 min a day for 5 days, cell growth under the 5-V stimulation was significantly suppressed as compared with the control culture. Expression of mRNAs encoding keratinocyte differentiation markers such as keratin 10, involucrin, transglutaminase 1, and filaggrin was significantly increased in response to the 5-V stimulation, while the 3-V stimulation induced no significant change. After the 5-V stimulation, enhanced immunofluorescent stainings of involucrin and filaggrin were observed. These results indicate that monodirectional pulsed electric stimulation induces the keratinocyte differentiation with growth arrest.
Indexed as
Identifiers
23564189What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.