ArticleApplied and environmental microbiology2013
Iron and copper act synergistically to delay anaerobic growth of bacteria.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Hydrogenase Mediated Biosynthesis of Catalytically Active Cu Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Iron, Copper, and Zinc Homeostasis in the Battle between Macrophage andCurrent medicinal chemistry · 2025Review
- The Critical Role of Environmental Synergies in the Creation of Bionanohybrid Microbes.Applied and environmental microbiology · 2022Article
- Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization.Environmental science. Nano · 2022Article
- Unveiling microbial preservation under hyperacidic and oxidizing conditions in the Oligocene Rio Tinto deposit.Scientific reports · 2021Article
- Identification and characterization of Cercospora beticola necrosis-inducing effector CbNip1.Molecular plant pathology · 2021Article
- Understanding gold toxicity in aerobically-grown Escherichia coli.Biological research · 2020Article
- Robust microorganisms for biofuel and chemical production from municipal solid waste.Microbial cell factories · 2020Article
- Zinc excess increases cellular demand for iron and decreases tolerance to copper inThe Journal of biological chemistry · 2019Article
- Predicting the decision making chemicals used for bacterial growth.Scientific reports · 2019Article
- Metabolic Inactivity and Re-awakening of a Nitrate Reduction Dependent Iron(II)-Oxidizing BacteriumFrontiers in microbiology · 2019Article
- Proteome Response of a Metabolically Flexible Anoxygenic Phototroph to Fe(II) Oxidation.Applied and environmental microbiology · 2018Article
- Survival of Anaerobic FeJournal of bacteriology · 2018Article
- Heterologous expression of a Streptomyces cyaneus laccase for biomass modification applications.AMB Express · 2017Article
- Transcriptomic analysis of aerobic respiratory and anaerobic photosynthetic states inMicrobial genomics · 2017Article
- Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli.Applied and environmental microbiology · 2017Article
- Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses.Scientific reports · 2017Article
- Timescales of Oxygenation Following the Evolution of Oxygenic Photosynthesis.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2016Article
- The Effect of Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin Solution on Growth and Volatile Sulfur Compounds Production of Oral Malodor Associated Some Anaerobic Periodontal Bacteria.Yonago acta medica · 2015Article
- Multidrug Efflux Systems in Microaerobic and Anaerobic Bacteria.Antibiotics (Basel, Switzerland) · 2015Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Transition metals are known to cause toxic effects through their interaction with oxygen, but toxicity under anoxic conditions is poorly understood. Here we investigated the effects of iron (Fe) and copper (Cu) on the anaerobic growth and gene expression of the purple phototrophic bacterium Rhodopseudomonas palustris TIE-1. We found that Fe(II) and Cu(II) act synergistically to delay anaerobic growth at environmentally relevant metal concentrations. Cu(I) and Cu(II) had similar effects both alone and in the presence of ascorbate, a Cu(II) reductant, indicating that reduction of Cu(II) to Cu(I) by Fe(II) is not sufficient to explain the growth inhibition. Addition of Cu(II) increased the toxicity of Co(II) and Ni(II); in contrast, Ni(II) toxicity was diminished in the presence of Fe(II). The synergistic anaerobic toxicity of Fe(II) and Cu(II) was also observed for Escherichia coli MG1655, Shewanella oneidensis MR-1, and Rhodobacter capsulatus SB1003. Gene expression analyses for R. palustris identified three regulatory genes that respond to Cu(II) and not to Fe(II): homologs of cueR and cusR, two known proteobacterial copper homeostasis regulators, and csoR, a copper regulator recently identified in Mycobacterium tuberculosis. Two P-type ATPase efflux pumps, along with an F(o)F(1) ATP synthase, were also upregulated by Cu(II) but not by Fe(II). An Escherichia coli mutant deficient in copA, cus, and cueO showed a smaller synergistic effect, indicating that iron might interfere with one or more of the copper homeostasis systems. Our results suggest that interactive effects of transition metals on microbial physiology may be widespread under anoxic conditions, although the molecular mechanisms remain to be more fully elucidated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.