ArticleApplied and environmental microbiology2013
Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- The Metabolic Reprogramming Mechanism by Pueraria Lobata Fermentation Enhances Saccharomyces cerevisiae Reducing-Sugar-Releasing Activity and Polypeptide Synthesis.Current microbiology · 2026Article
- Engineering fusion enzymes for enhanced catalysis and stability: strategies and biotechnological applications.Molecular biology reports · 2026Review
- Degradation-Suppressed Cocoonase for Investigating the Propeptide-Mediated Activation Mechanism.Molecules (Basel, Switzerland) · 2022Article
- Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.Microbial cell factories · 2020Article
- Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications.World journal of microbiology & biotechnology · 2017Review
- Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy.Scientific reports · 2016Article
- Improvement of catalytic efficiency and thermostability of recombinant Streptomyces griseus trypsin by introducing artificial peptide.World journal of microbiology & biotechnology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.