ArticleOsteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA2013
Poor histological healing of a femoral fracture following 12 months of oestrogen deficiency in rats.
Article in Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Analysis of low-dose estrogen on callus BMD as measured by pQCT in postmenopausal women.BMC musculoskeletal disorders · 2020Trial
- Fortified Withaferin A accelerates the transition from fibrovascular to bone remodeling phase during endochondral bone formation to promote ossification.Frontiers in endocrinology · 2025Article
- Bone regeneration during osteoporosis: a translationalFrontiers in bioengineering and biotechnology · 2025Article
- Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis.Lasers in medical science · 2023Article
- Fracture Healing in the Setting of Endocrine Diseases, Aging, and Cellular Senescence.Endocrine reviews · 2022Review
- Surgical Classification for Preclinical Rat Femoral Bone Defect Model: Standardization Based on Systematic Review, Anatomical Analysis and Virtual Surgery.Bioengineering (Basel, Switzerland) · 2022Review
- The effect of osteoporosis and its treatment on fracture healing a systematic review of animal and clinical studies.Bone reports · 2021Review
- Bosentan, a drug used in the treatment of pulmonary hypertension, can prevent development of osteoporosis.Iranian journal of basic medical sciences · 2021Article
- Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats.Experimental and therapeutic medicine · 2017Article
- The impact of low-magnitude high-frequency vibration on fracture healing is profoundly influenced by the oestrogen status in mice.Disease models & mechanisms · 2015Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
summaryFractures in post-menopausal osteoporosis cause significant morbidity; however, animal models for post-menopausal fracture healing lack the effect of ageing. Therefore, we developed a model using aged animals with chronic oestrogen deficiency, which demonstrates inferior fracture repair (decreased healing histologically, bone mineral density and content and strength). This novel model may help develop molecular strategies for osteoporotic fracture repair.
introductionThe femur is susceptible to damage by both systemic conditions such as osteoporosis and locally by traumatic injury. The capacity for fracture repair decreases with age, while the risk of fracture increases. As studies of osteoporotic fracture healing in rats traditionally use a period of 3 months or less of oestrogen deficiency prior to fracturing, we aimed to establish a osteoporosis model in rats with chronic oestrogen deficiency by 12 months to better mimic human female osteoporosis.
methodsSeventy female Sprague-Dawley rats (10 weeks old) were ovariectomised or sham operated and housed for 12 months. The right femur was fractured by way of an open osteotomy and fixed with an intramedullary Kirschner wire. Animals were sacrificed at 1, 3 and 6 weeks for radiography, dual-energy X-ray absorptiometry, tensile testing and histology.
resultsBone mineral density and bone mineral content were lower by 60 and 63 %, respectively, (p < 0.05) in the bilaterally ovariectomized (OVX) groups than those in the sham groups at 6 weeks in the right fractured femurs. Maximum breaking force of the OVX group was lower than that of the sham group, with the greatest difference seen at 6 weeks following osteotomy. Histologically, the OVX groups demonstrated a delay in cellular differentiation within the fracture callus and the presence of bone resorption. The sham animals had a superior histological healing pattern with an Allen score of 4 at 6 weeks compared to a score of 1 for the OVX groups (p < 0.01).
conclusionsLong-term ovariectomy has a deleterious effect on fracture healing in a rodent model.
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