ArticleThe Indian journal of medical research2013
Strategy for identification & characterization of Bartonella henselae with conventional & molecular methods.
Article in The Indian journal of medical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Molecular Detection ofPathogens (Basel, Switzerland) · 2026Article
- Uncovering the truth about cat-scratch disease.Open veterinary journal · 2025Review
- Article
- Epidemiology of Bartonella henselae infection in pet and stray cats in Croatia with risk factors analysis.Parasites & vectors · 2024Article
- Aetiologies of bacterial tick-borne febrile illnesses in humans in Africa: diagnostic limitations and the need for improvement.Frontiers in medicine · 2024Review
- Blood Supplementation Enhances Bartonella henselae Growth and Molecular Detection of Bacterial DNA in Liquid Culture.Microbiology spectrum · 2023Article
- Two novelFrontiers in microbiology · 2023Article
- Biomolecular Investigation ofPathogens (Basel, Switzerland) · 2021Article
- Isolation, characterization, and molecular identification of soil bacteria showing antibacterial activity against human pathogenic bacteria.Journal, genetic engineering & biotechnology · 2021Article
- Bartonella infections in cats and dogs including zoonotic aspects.Parasites & vectors · 2018Review
- Antibacterial activity of soil bacteria isolated from Kochi, India and their molecular identification.Journal, genetic engineering & biotechnology · 2018Article
- Article
- Cat-scratch disease: unusual perivascular chorioretinal lesions.Medical hypothesis, discovery & innovation ophthalmology journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesBartonella henselae is a fastidious gram-negative bacterium usually causing self limiting infections in immunocompetent individuals but often causes potentially life threatening infection, such as bacillary angiomatosis in immunocompromised patients. Both diagnosis of infections and research into molecular mechanisms of pathogenesis have been hindered by lack of appropriate and reliable diagnostic techniques. We undertook this study to standardize methods to characterize B. henselae in clinical samples to diagnose Bartonella infection correctly.
methodsB. henselae ATCC 49882 strain was procured from American type culture collection, USA. This strain was revived and maintained in the laboratory, and identification and characterization of this strain was done by conventional and molecular techniques, which included culture on various media, staining by different methods including electron microscopy, biochemical analysis by conventional methods and API, polymerase chain reaction (PCR) for amplification of citrate synthase gene followed by restriction fragment length polymorphism (RFLP).
resultsThis organism was biochemically inert due to slow growth and generated unique identification code with API. The amplification of the citrate-synthase gene with primers yielded a 381 bp product followed by specific RFLP profile for B. henselae. INTERPRETATION &
conclusionsBartonella is fastidious and fragile organism and should be handled carefully. Extra effort and careful observation are required to isolate and characterize this organism.
Indexed as
Identifiers
23563383PMC3657863W2133342984What OpenQuestion holds
Registered trials
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