ArticleJournal of structural biology2013
Localization and quantitative co-localization of enamelin with amelogenin.
Article in Journal of structural biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Identification of stages of amelogenesis in the continuously growing mandiblular incisor of C57BL/6J male mice throughout life using molar teeth as landmarks.Frontiers in physiology · 2023Article
- The phosphorylation of serineMatrix biology : journal of the International Society for Matrix Biology · 2022Article
- Biomineralization of Enamel and Dentin Mediated by Matrix Proteins.Journal of dental research · 2021Review
- Mapping and Identification of Native Proteins of Developing Teeth in Mouse Mandibles.Analytical chemistry · 2020Article
- Amelogenin phosphorylation regulates tooth enamel formation by stabilizing a transient amorphous mineral precursor.The Journal of biological chemistry · 2020Article
- Co-Immunoprecipitation Reveals Interactions Between Amelogenin and AmeloblastinFrontiers in physiology · 2020Article
- Proteolysis by MMP20 Prevents Aberrant Mineralization in Secretory Enamel.Journal of dental research · 2019Article
- Amelogenic transcriptome profiling in ameloblast-like cells derived from adult gingival epithelial cells.Scientific reports · 2019Article
- Ameloblastin Binds to Phospholipid Bilayers via a Helix-Forming Motif within the Sequence Encoded by Exon 5.ACS omega · 2019Article
- Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns.Frontiers in physiology · 2019Article
- Immunohistochemical Co-Localization of Amelogenin and Ameloblastin in Developing Enamel Matrix.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Control of Calcium Phosphate Nucleation and Transformation through Interactions of Enamelin and Amelogenin Exhibits the "Goldilocks Effect".Crystal growth & design · 2018Article
- Significance of genetic variations in developmental enamel defects of primary dentition in Polish children.Clinical oral investigations · 2018Article
- Ameloblastin peptide encoded by exon 5 interacts with amelogenin N-terminus.Biochemistry and biophysics reports · 2016Article
- Amelogenin-Ameloblastin Spatial Interaction around Maturing Enamel Rods.Journal of dental research · 2016Article
- Structural adaptation of tooth enamel protein amelogenin in the presence of SDS micelles.Biopolymers · 2014Article
- Helium ion microscopy of enamel crystallites and extracellular tooth enamel matrix.Frontiers in physiology · 2014Article
- Analysis of co-assembly and co-localization of ameloblastin and amelogenin.Frontiers in physiology · 2014Article
- Dental enamel development: proteinases and their enamel matrix substrates.ISRN dentistry · 2013Review
- Amelogenin and Enamel Biomimetics.Journal of materials chemistry. BArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Enamelin and amelogenin are vital proteins in enamel formation. The cooperative function of these two proteins controls crystal nucleation and morphology in vitro. We quantitatively analyzed the co-localization between enamelin and amelogenin by confocal microscopy and using two antibodies, one raised against a sequence in the porcine 32 kDa enamelin region and the other raised against full-length recombinant mouse amelogenin. We further investigated the interaction of the porcine 32 kDa enamelin and recombinant amelogenin using immuno-gold labeling. This study reports the quantitative co-localization results for postnatal days 1-8 mandibular mouse molars. We show that amelogenin and enamelin are secreted into the extracellular matrix on the cuspal slopes of the molars at day 1 and that secretion continues to at least day 8. Quantitative co-localization analysis (QCA) was performed in several different configurations using large (45 μm height, 33 μm width) and small (7 μm diameter) regions of interest to elucidate any patterns. Co-localization patterns in day 8 samples revealed that enamelin and amelogenin co-localize near the secretory face of the ameloblasts and appear to be secreted approximately in a 1:1 ratio. The degree of co-localization decreases as the enamel matures, both along the secretory face of ameloblasts and throughout the entire thickness of the enamel. Immuno-reactivity against enamelin is concentrated along the secretory face of ameloblasts, supporting the theory that this protein together with amelogenin is intimately involved in mineral induction at the beginning of enamel formation.
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Registered trials
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