ArticleParasitology international2013
Whole-cell patch-clamp recording of nicotinic acetylcholine receptors in adult Brugia malayi muscle.
Article in Parasitology international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Diethylcarbamazine elicits CaAntimicrobial agents and chemotherapy · 2023Article
- Reconstitution of an N-AChR from Brugia malayi, an evolved change in acetylcholine receptor accessory protein requirements in filarial parasites.PLoS pathogens · 2022Article
- Anthelmintic resistance and homeostatic plasticity (Brugia malayi).Scientific reports · 2021Article
- Ivermectin inhibits extracellular vesicle secretion from parasitic nematodes.Journal of extracellular vesicles · 2020Article
- Adapting techniques for calcium imaging in muscles of adult Brugia malayi.Invertebrate neuroscience : IN · 2020Article
- EAT-18 is an essential auxiliary protein interacting with the non-alpha nAChR subunit EAT-2 to form a functional receptor.PLoS pathogens · 2020Article
- Emodepside has sex-dependent immobilizing effects on adult Brugia malayi due to a differentially spliced binding pocket in the RCK1 region of the SLO-1 K channel.PLoS pathogens · 2019Article
- Functional genomics inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Expression of five acetylcholine receptor subunit genes in Brugia malayi adult worms.International journal for parasitology. Drugs and drug resistance · 2015Article
- Transient effects of levamisole on Brugia malayi microfilariae.Invertebrate neuroscience : IN · 2015Article
- The Conqueror Worm: recent advances with cholinergic anthelmintics and techniques excite research for better therapeutic drugs.Journal of helminthology · 2015Article
- Utilization of computer processed high definition video imaging for measuring motility of microscopic nematode stages on a quantitative scale: "The Worminator".International journal for parasitology. Drugs and drug resistance · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Lymphatic filariasis is a debilitating disease caused by clade III parasites like Brugia malayi and Wuchereria bancrofti. Current recommended treatment regimen for this disease relies on albendazole, ivermectin and diethylcarbamazine, none of which targets the nicotinic acetylcholine receptors in these parasitic nematodes. Our aim therefore has been to develop adult B. malayi for electrophysiological recordings to aid in characterizing the ion channels in this parasite as anthelmintic target sites. In that regard, we recently demonstrated the amenability of adult B. malayi to patch-clamp recordings and presented results on the single-channel properties of nAChR in this nematode. We have built on this by recording whole-cell nAChR currents from adult B. malayi muscle. Acetylcholine, levamisole, pyrantel, bephenium and tribendimidine activated the receptors on B. malayi muscle, producing robust currents ranging from >200 pA to ~1.5 nA. Levamisole completely inhibited motility of the adult B. malayi within 10 min and after 60 min, motility had recovered back to control values.
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