ArticleStem cell research2013
Generation of islet-like cells from mouse gall bladder by direct ex vivo reprogramming.
Article in Stem cell research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Stomach-derived human insulin-secreting organoids restore glucose homeostasis.Nature cell biology · 2023Article
- Direct Reprogramming of Somatic Cells into Induced β-Cells: An Overview.Advances in experimental medicine and biology · 2023Article
- Direct Reprogramming of Different Cell Lineages into Pancreatic β-Like Cells.Cellular reprogramming · 2022Review
- Single-cell transcriptome and accessible chromatin dynamics during endocrine pancreas development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Pro-Endocrine Pancreatic Islet Transcriptional Program Established During Development Is Retained in Human Gallbladder Epithelial Cells.Cellular and molecular gastroenterology and hepatology · 2022Article
- PDX1 is the cornerstone of pancreatic β-cell functions and identity.Frontiers in molecular biosciences · 2022Review
- Transcription Factor Reprogramming in the Inner Ear: Turning on Cell Fate Switches to Regenerate Sensory Hair Cells.Frontiers in cellular neuroscience · 2021Article
- Generation of insulin-secreting cells from mouse gallbladder stem cells by small molecules in vitro.Stem cell research & therapy · 2019Article
- Reprogramming Cells to Make Insulin.Journal of the Endocrine Society · 2019Review
- Long-Term Correction of Diabetes in Mice by In Vivo Reprogramming of Pancreatic Ducts.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Elixir of Life: Thwarting Aging With Regenerative Reprogramming.Circulation research · 2018Review
- PDX1, Neurogenin-3, and MAFA: critical transcription regulators for beta cell development and regeneration.Stem cell research & therapy · 2017Review
- Regenerative medicine and cell-based approaches to restore pancreatic function.Nature reviews. Gastroenterology & hepatology · 2017Review
- Article
- In vivo functional and morphological characterization of bone and striated muscle microcirculation in NSG mice.PloS one · 2017Article
- Pancreatic islet cell development and regeneration.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- Novel surface markers directed against adult human gallbladder.Stem cell research · 2015Article
- Reprogramming Mouse Cells With a Pancreatic Duct Phenotype to Insulin-Producing β-Like Cells.Endocrinology · 2015Article
- Identification of a candidate stem cell in human gallbladder.Stem cell research · 2015Article
- Direct Reprogramming for Pancreatic Beta-Cells Using Key Developmental Genes.Current pathobiology reports · 2015Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Cell replacement is an emerging therapy for type 1 diabetes. Pluripotent stem cells have received a lot of attention as a potential source of transplantable β-cells, but their ability to form teratomas poses significant risks. Here, we evaluated the potential of primary mouse gall bladder epithelial cells (GBCs) as targets for ex vivo genetic reprogramming to the β-cell fate. Conditions for robust expansion and genetic transduction of primary GBCs by adenoviral vectors were developed. Using a GFP reporter for insulin, conditions for reprogramming were then optimized. Global expression analysis by RNA-sequencing was used to quantitatively compare reprogrammed GBCs (rGBCs) to true β-cells, revealing both similarities and differences. Adenoviral-mediated expression of NEUROG3, Pdx1, and MafA in GBCs resulted in robust induction of pancreatic endocrine genes, including Ins1, Ins2, Neurod1, Nkx2-2 and Isl1. Furthermore, expression of GBC-specific genes was repressed, including Sox17 and Hes1. Reprogramming was also enhanced by addition of retinoic acid and inhibition of Notch signaling. Importantly, rGBCs were able to engraft long term in vivo and remained insulin-positive for 15weeks. We conclude that GBCs are a viable source for autologous cell replacement in diabetes, but that complete reprogramming will require further manipulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.