ArticleExperimental eye research2013
Retinal deimination and PAD2 levels in retinas from donors with age-related macular degeneration (AMD).
Article in Experimental eye research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Role of the PADI family in inflammatory autoimmune diseases and cancers: A systematic review.Frontiers in immunology · 2023Pooled it
- Unveiling the Web: Exploring the Multifaceted Role of Neutrophil Extracellular Traps in Ocular Health and Disease.Journal of clinical medicine · 2024Review
- Article
- A corneo-retinal hypercitrullination axis underlies ocular injury to nitrogen mustard.Experimental eye research · 2023Article
- Peptidyl arginine deiminase 4 deficiency protects against subretinal fibrosis by inhibiting Müller glial hypercitrullination.Journal of neuroscience research · 2023Article
- Histopathological assessments reveal retinal vascular changes, inflammation, and gliosis in patients with lethal COVID-19.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2022Article
- Compartmentalized citrullination in Muller glial endfeet during retinal degeneration.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Targeting the integrated stress response in ophthalmology.Current eye research · 2021Review
- Histopathological assessments reveal retinal vascular changes, inflammation and gliosis in patients with lethal COVID-19.medRxiv : the preprint server for health sciences · 2021Article
- Evidence of complement dysregulation in outer retina of Stargardt disease donor eyes.Redox biology · 2020Article
- Article
- Murine Retinal Citrullination Declines With Age and is Mainly Dependent on Peptidyl Arginine Deiminase 4 (PAD4).Investigative ophthalmology & visual science · 2018Article
- Perspective on AMD Pathobiology: A Bioenergetic Crisis in the RPE.Investigative ophthalmology & visual science · 2018Review
- Sustaining Intravitreal Residence With L-Arginine Peptide-Conjugated Nanocarriers.Investigative ophthalmology & visual science · 2017Article
- Expression of peptidylarginine deiminase 4 in an alkali injury model of retinal gliosis.Biochemical and biophysical research communications · 2017Article
- Citrullination of glial intermediate filaments is an early response in retinal injury.Molecular vision · 2016Article
- DJ-1-dependent regulation of oxidative stress in the retinal pigment epithelium (RPE).PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Deimination is a form of protein posttranslational modification carried out by the peptidyl arginine deiminases (PADs) enzymes. PAD2 is the principal deiminase expressed in the retina. Elevated levels of PAD2 and protein deimination are present in a number of human neurological diseases, with or without ocular manifestation. To define the association of deimination with the pathogenesis of age-related macular degeneration (AMD), we studied protein deimination and PAD2 levels in retinas of AMD donor eyes compared to age-matched non-AMD retinas. Eyes from non-AMD and AMD donors were fixed in 4% paraformaldehyde and 0.5% glutaraldehyde in phosphate buffer. Retina and retinal pigment epithelium (RPE) from donor eyes were processed for immunohistochemical detection and western blotting using antibodies to PAD2 and citrulline residues. The ganglion cell, inner plexiform, inner nuclear and outer nuclear layers were labeled by both PAD2 and citrulline antibodies. Changes in the localization of deiminated residues and PAD2 were evident as the retinal layers were remodeled coincident with photoreceptor degeneration in AMD retinas. Immunodetection of either PAD2 or citrulline residues could not be evaluated in the RPE layer due to the high autofluorescence levels in this layer. Interestingly, higher deimination immunoreactivity was detected in AMD retinal lysates. However, no significant changes in PAD2 were detected in the AMD and non-AMD retinas and RPE lysates. Our observations show increased levels of protein deimination but not PAD2 in AMD retinas and RPE, suggesting a reduced rate of turnover of deiminated proteins in these AMD retinas.
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Registered trials
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