ArticleBiophysical journal2013
Mechanical properties of doubly stabilized microtubule filaments.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 88 citations in OpenAlex.
- Tau-Driven Coordination of Microtubule-Actin Crosstalk in Cell-Sized Vesicles.bioRxiv : the preprint server for biology · 2025Article
- Preparation of Polarity-Marked Microtubules Using a Plus-End Capping DARPin.Bio-protocol · 2024Article
- The structure of a Tau fragment bound to tubulin prompts new hypotheses on Tau mechanism and oligomerization.PNAS nexus · 2024Article
- Effects of three microtubule-associated proteins (MAP2, MAP4, and Tau) on microtubules' physical properties and neurite morphology.Scientific reports · 2023Article
- Nitric oxide: A core signaling molecule under elevated GHGs (COFrontiers in plant science · 2022Review
- Growth rate-dependent flexural rigidity of microtubules influences pattern formation in collective motion.Journal of nanobiotechnology · 2021Article
- Primary cilium: a paradigm for integrating mathematical modeling with experiments and numerical simulations in mechanobiology.Mathematical biosciences and engineering : MBE · 2021Article
- C-Terminal Tail Polyglycylation and Polyglutamylation Alter Microtubule Mechanical Properties.Biophysical journal · 2020Article
- Microtubule instability driven by longitudinal and lateral strain propagation.PLoS computational biology · 2020Article
- Coarse-Grained Simulation of Mechanical Properties of Single Microtubules With Micrometer Length.Frontiers in molecular biosciences · 2020Article
- Microtubule stabilizer reveals requirement of CaBiology open · 2019Article
- A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel.The Journal of general physiology · 2019Review
- Microtubule structure by cryo-EM: snapshots of dynamic instability.Essays in biochemistry · 2018Review
- Microtubule-Based Control of Motor-Clutch System Mechanics in Glioma Cell Migration.Cell reports · 2018Article
- Tau can switch microtubule network organizations: from random networks to dynamic and stable bundles.Molecular biology of the cell · 2018Article
- Label-free Imaging and Bending Analysis of Microtubules by ROCS Microscopy and Optical Trapping.Biophysical journal · 2018Article
- Interplay of structure, elasticity, and dynamics in actin-based nematic materials.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
- Manipulation and quantification of microtubule lattice integrity.Biology open · 2017Article
- High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.Cytoskeleton (Hoboken, N.J.) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules are cytoskeletal filaments responsible for cell morphology and intracellular organization. Their dynamical and mechanical properties are regulated through the nucleotide state of the tubulin dimers and the binding of drugs and/or microtubule-associated proteins. Interestingly, microtubule-stabilizing factors have differential effects on microtubule mechanics, but whether stabilizers have cumulative effects on mechanics or whether one effect dominates another is not clear. This is especially important for the chemotherapeutic drug Taxol, an important anticancer agent and the only known stabilizer that reduces the rigidity of microtubules. First, we ask whether Taxol will combine additively with another stabilizer or whether one stabilizer will dominate another. We call microtubules in the presence of Taxol and another stabilizer, doubly stabilized. Second, since Taxol is often added to a number of cell types for therapeutic purposes, it is important from a biomedical perspective to understand how Taxol added to these systems affects the mechanical properties in treated cells. To address these questions, we use the method of freely fluctuating filaments with our recently developed analysis technique of bootstrapping to determine the distribution of persistence lengths of a large population of microtubules treated with different stabilizers, including Taxol, guanosine-5' [(α, β)-methyleno] triphosphate, guanosine-5'-O-(3-thiotriphosphate), tau, and MAP4. We find that combinations of these stabilizers have novel effects on the mechanical properties of microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.