ArticleBiophysical journal2013
Phase diagram and tie-line determination for the ternary mixture DOPC/eSM/cholesterol.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed.
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- Cholesterol and Lipid Rafts in the Biogenesis of Amyloid-β Protein and Alzheimer's Disease.Annual review of biophysics · 2024Review
- Multispherical shapes of vesicles with intramembrane domains.The European physical journal. E, Soft matter · 2024Article
- Secondary Ion Mass Spectrometry of Single Giant Unilamellar Vesicles Reveals Compositional Variability.Journal of the American Chemical Society · 2023Article
- Dynamics of asymmetric membranes and interleaflet coupling as intermediates in membrane fusion.Biophysical journal · 2023Article
- Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).Biophysical journal · 2023Article
- Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins.iScience · 2023Article
- Contacting domains segregate a lipid transporter from a solute transporter in the malarial host-parasite interface.Nature communications · 2020Article
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour.Micromachines · 2020Article
- Observations of Membrane Domain Reorganization in Mechanically Compressed Artificial Cells.Chembiochem : a European journal of chemical biology · 2019Article
- Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.Biophysical journal · 2019Article
- Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.Biophysical journal · 2018Article
- Charged giant unilamellar vesicles prepared by electroformation exhibit nanotubes and transbilayer lipid asymmetry.Scientific reports · 2018Article
- FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells.Biophysical journal · 2018Article
- Membrane Bending Moduli of Coexisting Liquid Phases Containing Transmembrane Peptide.Biophysical journal · 2018Article
- Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Janus dendrimersomes coassembled from fluorinated, hydrogenated, and hybrid Janus dendrimers as models for cell fusion and fission.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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5 authors.
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Abstract
We propose a novel, to our knowledge, method for the determination of tie lines in a phase diagram of ternary lipid mixtures. The method was applied to a system consisting of dioleoylphosphatidylcholine (DOPC), egg sphingomyelin (eSM), and cholesterol (Chol). The approach is based on electrofusion of single- or two-component homogeneous giant vesicles in the fluid phase and analyses of the domain areas of the fused vesicle. The electrofusion approach enables us to create three-component vesicles with precisely controlled composition, in contrast to conventional methods for giant vesicle formation. The tie lines determined in the two-liquid-phase coexistence region are found to be not parallel, suggesting that the dominant mechanism of lipid phase separation in this region changes with the membrane composition. We provide a phase diagram of the DOPC/eSM/Chol mixture and predict the location of the critical point. Finally, we evaluate the Gibbs free energy of transfer of individual lipid components from one phase to the other.
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