ReviewBiophysical journal2013
Evanescent excitation and emission in fluorescence microscopy.
Review in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles.Nature communications · 2023Article
- Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation.Bioscience reports · 2022Review
- Micro- and Nanoscale Imaging of Fluids in Water Using Refractive-Index-Matched Materials.Nanomaterials (Basel, Switzerland) · 2022Review
- Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking.International journal of molecular sciences · 2022Article
- upU-Net Approaches for Background Emission Removal in Fluorescence Microscopy.Journal of imaging · 2022Article
- Light scattering in TIRF microscopy: A theoretical study of the limits to surface selectivity.Biophysical journal · 2021Article
- Single-shot, shadowless total internal reflection fluorescence microscopy via annular fiber bundle.Optics letters · 2020Article
- Supercritical Angle Fluorescence Microscopy and Spectroscopy.Biophysical journal · 2020Review
- Fluorescence imaging with tailored light.Nanophotonics (Berlin, Germany) · 2019Article
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.The Journal of general physiology · 2019Review
- Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization.Biomedical optics express · 2019Article
- Combining 3D single molecule localization strategies for reproducible bioimaging.Nature communications · 2019Article
- Article
- Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2017Article
- Near-Membrane Refractometry Using Supercritical Angle Fluorescence.Biophysical journal · 2017Article
- Cascaded plasmon-plasmon coupling mediated energy transfer across stratified metal-dielectric nanostructures.Scientific reports · 2016Article
- Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.Biophysical journal · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
1 author.
Funding
Abstract
Evanescent light-light that does not propagate but instead decays in intensity over a subwavelength distance-appears in both excitation (as in total internal reflection) and emission (as in near-field imaging) forms in fluorescence microscopy. This review describes the physical connection between these two forms as a consequence of geometrical squeezing of wavefronts, and describes newly established or speculative applications and combinations of the two. In particular, each can be used in analogous ways to produce surface-selective images, to examine the thickness and refractive index of films (such as lipid multilayers or protein layers) on solid supports, and to measure the absolute distance of a fluorophore to a surface. In combination, the two forms can further increase selectivity and reduce background scattering in surface images. The polarization properties of each lead to more sensitive and accurate measures of fluorophore orientation and membrane micromorphology. The phase properties of the evanescent excitation lead to a method of creating a submicroscopic area of total internal reflection illumination or enhanced-resolution structured illumination. Analogously, the phase properties of evanescent emission lead to a method of producing a smaller point spread function, in a technique called virtual supercritical angle fluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.