ArticleThe journal of physical chemistry. B2013
A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution.
Article in The journal of physical chemistry. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Nuclear-Spin Hyperpolarization of Biomolecules by Optically Enhanced NMR at Ultra-High Field.Journal of the Chinese Chemical Society. Zhongguo hua xue hui (Taipei, Taiwan) · 2026Article
- Continuous-Wave (CW) Photo-CIDNP NMR Spectroscopy: A Tutorial.Magnetic resonance in chemistry : MRC · 2025Article
- Highly Sensitive Detection of Tyrosine and Neurotransmitters by Stereoselective Biosynthesis and Photochemically Induced Dynamic Nuclear Polarization.Journal of the American Chemical Society · 2025Article
- Photo-CIDNP for quantification of micromolar analytes in urine.Communications chemistry · 2025Article
- Investigation of Methionine Metabolism in Coccolithophore byThe journal of physical chemistry letters · 2025Article
- LC-Photo-CIDNP hyperpolarization of biomolecules bearing a quasi-isolated spin pair: Magnetic-Field dependence via a rapid-shuttling device.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- Ultrafast Fragment Screening Using Photo-Hyperpolarized (CIDNP) NMR.Journal of the American Chemical Society · 2023Article
- Spin Hyperpolarization in Modern Magnetic Resonance.Chemical reviews · 2023Review
- Magnetic-Field Dependence of LC-Photo-CIDNP in the Presence of Target Molecules Carrying a Quasi-Isolated Spin Pair.Applied magnetic resonance · 2023Article
- Sample illumination device facilitates in situ light-coupled NMR spectroscopy without fibre optics.Communications chemistry · 2022Article
- Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration.Journal of the American Chemical Society · 2022Article
- Enhanced nuclear-spin hyperpolarization of amino acids and proteins via reductive radical quenchers.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2021Article
- Exploration of the close chemical space of tryptophan and tyrosine reveals importance of hydrophobicity in CW-photo-CIDNP performances.Magnetic resonance (Gottingen, Germany) · 2021Article
- Fast-pulsing LED-enhanced NMR: A convenient and inexpensive approach to increase NMR sensitivity.The Journal of chemical physics · 2019Article
- Improved sensitivity of laser-enhancedJournal of magnetic resonance (San Diego, Calif. : 1997) · 2019Article
- Laser- and cryogenic probe-assisted NMR enables hypersensitive analysis of biomolecules at submicromolar concentration.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Pushing nuclear magnetic resonance sensitivity limits with microfluidics and photo-chemically induced dynamic nuclear polarization.Nature communications · 2018Article
- Effect of heavy atoms on photochemically induced dynamic nuclear polarization in liquids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Fluorescein: A Photo-CIDNP Sensitizer Enabling Hypersensitive NMR Data Collection in Liquids at Low Micromolar Concentration.The journal of physical chemistry. B · 2016Article
- Sensitivity enhancement in solution NMR: emerging ideas and new frontiers.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
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2 authors.
Funding
Abstract
NMR is an extremely powerful, yet insensitive technique. Many available nuclear polarization methods that address sensitivity are not directly applicable to low-concentration biomolecules in liquids and are often too invasive. Photochemically induced dynamic nuclear polarization (photo-CIDNP) is no exception. It needs high-power laser irradiation, which often leads to sample degradation, and photosensitizer reduction. Here, we introduce a novel tri-enzyme system that significantly overcomes the above challenges, rendering photo-CIDNP a practically applicable technique for NMR sensitivity enhancement in solution. The specificity of the nitrate reductase (NR) enzyme is exploited to selectively in situ reoxidize the reduced photo-CIDNP dye FMNH2. At the same time, the oxygen-scavenging ability of glucose oxidase (GO) and catalase (CAT) is synergistically employed to prevent sample photodegradation. The resulting tri-enzyme system (NR-GO-CAT) enables prolonged sensitivity-enhanced data collection in 1D and 2D heteronuclear NMR, leading to the highest photo-CIDNP sensitivity enhancement (48-fold relative to SE-HSQC) achieved to date for amino acids and polypeptides in solution. NR-GO-CAT extends the concentration limit of photo-CIDNP NMR down to the low micromolar range. In addition, sensitivity (relative to the reference SE-HSQC) is found to be inversely proportional to sample concentration, paving the way for the future analysis of even more diluted samples.
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