ArticleTissue engineering. Part C, Methods2013
Efficient generation of functional epithelial and epidermal cells from human pluripotent stem cells under defined conditions.
Article in Tissue engineering. Part C, Methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications.Stem cell reviews and reports · 2024Pooled it
- Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing.World journal of stem cells · 2023Article
- Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.Journal of nanobiotechnology · 2022Article
- Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.International journal of biological sciences · 2020Article
- Effect of inoculum density on human-induced pluripotent stem cell expansion in 3D bioreactors.Cell proliferation · 2019Article
- Effect of the sonic hedgehog inhibitor GDC-0449 on an in vitro isogenic cellular model simulating odontogenic keratocysts.International journal of oral science · 2019Article
- Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives.Stem cells international · 2019Review
- Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells.Science advances · 2017Article
- Compliant substratum guides endothelial commitment from human pluripotent stem cells.Science advances · 2017Article
- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small-Molecule Inhibitor of Src Family Kinases.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells.Fluids and barriers of the CNS · 2015Article
- Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.Nature protocols · 2015Article
- Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling.Stem cell reports · 2014Article
- Improving efficiency of human pluripotent stem cell differentiation platforms using an integrated experimental and computational approach.Biotechnology and bioengineering · 2013Article
- Building a microphysiological skin model from induced pluripotent stem cells.Stem cell research & therapy · 2013Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Human pluripotent stem cells (hPSCs) have an unparalleled potential to generate limitless quantities of any somatic cell type. However, current methods for producing populations of various somatic cell types from hPSCs are generally not standardized and typically incorporate undefined cell culture components often resulting in variable differentiation efficiencies and poor reproducibility. To address this, we have developed a defined approach for generating epithelial progenitor and epidermal cells from hPSCs. In doing so, we have identified an optimal starting cell density to maximize yield and maintain high purity of K18+/p63+ simple epithelial progenitors. In addition, we have shown that the use of synthetic, defined substrates in lieu of Matrigel and gelatin can successfully facilitate efficient epithelial differentiation, maintaining a high (>75%) purity of K14+/p63+ keratinocyte progenitor cells and at a two to threefold higher yield than a previously reported undefined differentiation method. These K14+/p63+ cells also exhibited a higher expansion potential compared to cells generated using an undefined differentiation protocol and were able to terminally differentiate and recapitulate an epidermal tissue architecture in vitro. In summary, we have demonstrated the production of populations of functional epithelial and epidermal cells from multiple hPSC lines using a new, completely defined differentiation strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.