ArticlePloS one2013
U-shape suppressive effect of phenol red on the epileptiform burst activity via activation of estrogen receptors in primary hippocampal culture.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
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- TMEM97/Sigma 2 Receptor Increases Estrogen Receptor α Activity in Promoting Breast Cancer Cell Growth.Cancers · 2023Article
- Furosemide prevents membrane KCC2 downregulation during convulsant stimulation in the hippocampus.IBRO neuroscience reports · 2022Article
- A framework for developing sex-specific engineered heart models.Nature reviews. Materials · 2022Review
- Human-Induced Pluripotent Stem Cell-Based Models for Studying Sex-Specific Differences in Neurodegenerative Diseases.Advances in experimental medicine and biology · 2022Review
- Organoid technology in female reproductive biomedicine.Reproductive biology and endocrinology : RB&E · 2020Review
- Sex-Dependent Effects of 2,2',3,5',6-Pentachlorobiphenyl on Dendritic Arborization of Primary Mouse Neurons.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- 27-Hydroxycholesterol Induces Aberrant Morphology and Synaptic Dysfunction in Hippocampal Neurons.Cerebral cortex (New York, N.Y. : 1991) · 2019Article
- SK channels participate in the formation of after burst hyperpolarization and partly inhibit the burst strength of epileptic ictal discharges.Molecular medicine reports · 2018Article
- In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons.Scientific reports · 2017Article
- KCC2 downregulation facilitates epileptic seizures.Scientific reports · 2017Article
- Estrogen and phenol red free medium for osteoblast culture: study of the mineralization ability.Cytotechnology · 2016Article
- Effect of the PGD2-DP signaling pathway on primary cultured rat hippocampal neuron injury caused by aluminum overload.Scientific reports · 2016Article
- Colloidal drug formulations can explain "bell-shaped" concentration-response curves.ACS chemical biology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenol red is widely used in cell culture as a pH indicator. Recently, it also has been reported to have estrogen-like bioactivity and be capable of promoting cell proliferation in different cell lines. However, the effect of phenol red on primary neuronal culture has never been investigated. By using patch clamp technique, we demonstrated that hippocampal pyramidal neurons cultured in neurobasal medium containing no phenol red had large depolarization-associated epileptiform bursting activities, which were rarely seen in neurons cultured in phenol red-containing medium. Further experiment data indicate that the suppressive effect of the phenol red on the abnormal epileptiform burst neuronal activities was U-shape dose related, with the most effective concentration at 28 µM. In addition, this concentration related inhibitory effect of phenol red on the epileptiform neuronal discharges was mimicked by 17-β-estradiol, an estrogen receptor agonist, and inhibited by ICI-182,780, an estrogen receptor antagonist. Our results suggest that estrogen receptor activation by phenol red in the culture medium prevents formation of abnormal, epileptiform burst activity. These studies highlight the importance of phenol red as estrogen receptor stimulator and cautions of careful use of phenol red in cell culture media.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.