ArticlePloS one2013
Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Marked for Success: How RNA mCellular and molecular gastroenterology and hepatology · 2026Review
- Ceramide homeostasis in hepatic lipid droplets.Biochemical Society transactions · 2025Review
- Frontal Cortex Lipid Alterations During the Onset of Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2024Article
- Spatial transcriptomics add a new dimension to our understanding of the gut.American journal of physiology. Gastrointestinal and liver physiology · 2023Review
- Albino mice with the point mutation at the tyrosinase locus show high cholesterol diet-induced NASH susceptibility.Scientific reports · 2021Article
- SEAM is a spatial single nuclear metabolomics method for dissecting tissue microenvironment.Nature methods · 2021Article
- Expression of genes related to lipid transport in meat-type ducks divergent for low or high residual feed intake.Asian-Australasian journal of animal sciences · 2020Article
- Lipid mechanisms in hallmarks of cancer.Molecular omics · 2020Review
- Diet Induced Obesity Alters Intestinal Cytoplasmic Lipid Droplet Morphology and Proteome in the Postprandial Response to Dietary Fat.Frontiers in physiology · 2019Article
- Abnormality of intestinal cholesterol absorption inInternational journal of clinical and experimental pathology · 2019Article
- Imaging the distribution of skin lipids and topically applied compounds in human skin using mass spectrometry.Scientific reports · 2018Article
- Dual polarity MALDI imaging mass spectrometry on the same pixel points reveals spatial lipid localizations at high-spatial resolutions in rat small intestine.Analytical methods : advancing methods and applications · 2018Article
- Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution.Nature communications · 2017Article
- Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Shotgun Lipidomics Approach to Stabilize the Regiospecificity of Monoglycerides Using a Facile Low-Temperature Derivatization Enabling Their Definitive Identification and Quantitation.Analytical chemistry · 2016Article
- Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes.Biochimica et biophysica acta · 2016Review
- Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane.Nutrition & metabolism · 2016Article
- Perilipin-2 Modulates Lipid Absorption and Microbiome Responses in the Mouse Intestine.PloS one · 2015Article
- Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.PloS one · 2015Article
- Diacylglycerol acyltransferase-2 (DGAT2) and monoacylglycerol acyltransferase-2 (MGAT2) interact to promote triacylglycerol synthesis.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal absorption of dietary fat is a complex process mediated by enterocytes leading to lipid assembly and secretion of circulating lipoproteins as chylomicrons, vLDL and intestinal HDL (iHDL). Understanding lipid digestion is of importance knowing the correlation between excessive fat absorption and atherosclerosis. By using time-of-flight secondary ion mass spectrometry (TOF-SIMS), we illustrated a spatio-temporal localization of fat in mice duodenum, at different times of digestion after a lipid gavage, for the first time. Fatty acids progressively increased in enterocytes as well as taurocholic acid, secreted by bile and engaged in the entero-hepatic re-absorption cycle. Cytosolic lipid droplets (CLD) from enterocytes were originally purified separating chylomicron-like, intermediate droplets and smaller HDL-like. A lipidomic quantification revealed their contents in triglycerides, free and esterified cholesterol, phosphatidylcholine, sphingomyelin and ceramides but also in free fatty acids, mono- and di-acylglycerols. An acyl-transferase activity was identified and the enzyme monoacylglycerol acyl transferase 2 (MGAT2) was immunodetected in all CLD. The largest droplets was also shown to contain the microsomal triglyceride transfer protein (MTTP), the acyl-coenzyme A-cholesterol acyltransferases (ACAT) 1 and 2, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL). This highlights the fact that during the digestion of fats, enterocyte CLD contain some enzymes involved in the different stages of the metabolism of diet fatty acids and cholesterol, in anticipation of the crucial work of endoplasmic reticulum in the process. The data further underlines the dual role of chylomicrons and iHDL in fat digestion which should help to efficiently complement lipid-lowering therapy.
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