ArticleMolecular and cellular biology1990
Transcription of the rat and mouse proenkephalin genes is initiated at distinct sites in spermatogenic and somatic cells.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 107 citations in OpenAlex.
- Regulation of male fertility by the opioid system.Molecular medicine (Cambridge, Mass.) · 2011Review
- Gene expression of transcription factors in the rat brain after morphine withdrawal.Neurochemical research · 2004Article
- Widespread expression and estrogen regulation of PPEIA-3' nuclear RNA in the rat brain.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Interpreting cDNA sequences: some insights from studies on translation.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Review
- An alternatively spliced form of the transcription factor Sp1 containing only a single glutamine-rich transactivation domain.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The transcriptional regulation of the preproenkephalin gene.The Biochemical journal · 1995Review
- Structure and expression of the guinea pig preproenkephalin gene: site-specific cleavage in the 3' untranslated region yields truncated mRNA transcripts in specific brain regions.Molecular and cellular biology · 1995Article
- Heterogeneity in the 5' untranslated region of mouse cytochrome cT mRNAs leads to altered translational status of the mRNAs.Nucleic acids research · 1994Article
- Preproenkephalin promoter yields region-specific and long-term expression in adult brain after direct in vivo gene transfer via a defective herpes simplex viral vector.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Protein-DNA interactions associated with the onset of testis-specific expression of the mammalian Pgk-2 gene.Molecular and cellular biology · 1992Article
- An analysis of vertebrate mRNA sequences: intimations of translational control.The Journal of cell biology · 1991Review
- Localization of pre-messenger RNA at discrete nuclear sites.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1991Article
- Proenkephalin products are stored in the sperm acrosome and may function in fertilization.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
During spermatogenesis, several genes are expressed in a germ cell-specific manner. Previous studies have demonstrated that rat and mouse spermatogenic cells produce a 1,700-nucleotide proenkephalin RNA, while somatic cells that express the proenkephalin gene contain a 1,450-nucleotide transcript. Using cDNA cloning, RNA protection, and primer extension analyses, we showed that transcription of the rat and mouse spermatogenic-cell RNAs is initiated downstream from the proenkephalin somatic promoter in the first somatic intron (intron As). In both species, the germ cell cap site region consists of multiple start sites distributed over a length of approximately 30 base pairs. Within rat and mouse intron As, the region upstream of the germ cell cap sites is GC rich and lacks TATA sequences. A consensus binding site for the transcription factor SP1 was identified in intron As downstream of the proenkephalin germ cell cap site region. These features are characteristic of several previously described promoters that lack TATA sequences. Homologies were also identified between the proenkephalin and rat cytochrome c spermatogenic-cell promoters, including the absence of a TATA box, a multiple start site region, and several common sequences. This promoter motif thus may be shared with other genes expressed in male germ cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.