ArticleNature protocols2013
Disposable polymeric cryogel bioreactor matrix for therapeutic protein production.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Ice Templated PEG-Alginate Double-Network Cryogels with Tunable Mechanics and Degradation for Soft Tissue Engineering.Gels (Basel, Switzerland) · 2026Article
- Properties and Characterization of Cryogels: Structural, Mechanical, and Functional Insights.ACS omega · 2025Review
- Macroporous PEG-Alginate Hybrid Double-Network Cryogels with Tunable Degradation Rates Prepared via Radical-Free Cross-Linking for Cartilage Tissue Engineering.ACS applied bio materials · 2024Article
- Molecularly Imprinted Cryogels for the Selective Adsorption of Salicylic Acid.Applied biochemistry and biotechnology · 2023Article
- Interaction of haemin with albumin-based macroporous cryogel: Adsorption isotherm and fluorescence quenching studies.Frontiers in bioengineering and biotechnology · 2022Article
- Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water.Polymers · 2021Review
- Trends in Monoclonal Antibody Production Using Various Bioreactor Syst.Journal of microbiology and biotechnology · 2021Review
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
- Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery.Pharmaceutics · 2019Article
- A simple method for the production of large volume 3D macroporous hydrogels for advanced biotechnological, medical and environmental applications.Scientific reports · 2016Article
- Gelatin-Based Materials in Ocular Tissue Engineering.Materials (Basel, Switzerland) · 2014Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low cost and high efficiency make disposable bioreactors feasible for small-scale therapeutic development and initial clinical trials. We have developed a cryogel-based disposable bioreactor matrix, which has been used for production of protein therapeutics such as urokinase and monoclonal antibodies (mAbs). The protocol discusses the application of a cryogel bioreactor for mAb production. Cryogels composed of either polyacrylamide (PAAm) coupled to gelatin or semi-interpenetrating PAAm-chitosan are synthesized by free-radical polymerization at -12 °C. Hybridoma cells are immobilized over the cryogel bioreactor and incubated for 48 h. Medium is circulated thereafter at 0.2 ml min(-1) and bioreactors can be run continuously for 60 d. The cryogel-based packed-bed bioreactor can be formulated as a monolith or as beads; it also has an efficiency four times what can be obtained using a tissue-culture flask, a high surface-to-volume ratio and effective nutrient transport. After incubation, the bioreactor setup will take about 60 min using a pre-prepared sterilized cryogel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.