ArticleThe Journal of biological chemistry2013
Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 85 citations in OpenAlex.
- One-Pot Enzymatic ADDing of Click Chemistry Handles for Protein Immobilization and Bioconjugation of Small and Biomolecules.Bioconjugate chemistry · 2026Article
- The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea.Applied microbiology and biotechnology · 2025Review
- Flavin transferase ApbE: From discovery to applications.The Journal of biological chemistry · 2025Review
- Functional Characterization of RseC in the SoxR Reducing System and Its Role in Oxidative Stress Response inJournal of microbiology and biotechnology · 2024Article
- Versatile roles of protein flavinylation in bacterial extracyotosolic electron transfer.mSystems · 2024Article
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- Fixing Flavins: Hijacking a Flavin Transferase for Equipping Flavoproteins with a Covalent Flavin Cofactor.Journal of the American Chemical Society · 2023Article
- Promiscuous Enzymes for Residue-Specific Peptide and Protein Late-Stage Functionalization.Chembiochem : a European journal of chemical biology · 2023Review
- Article
- Characterization of two bacterial multi-flavinylated proteins harboring multiple covalent flavin cofactors.BBA advances · 2023Article
- A Novel, NADH-Dependent Acrylate Reductase in Vibrio harveyi.Applied and environmental microbiology · 2022Article
- Broadening the Scope of the Flavin-Tag Method by Improving Flavin Incorporation and Incorporating Flavin Analogs.Chembiochem : a European journal of chemical biology · 2022Article
- Inhibition of bacterial FMN transferase: A potential avenue for countering antimicrobial resistance.Protein science : a publication of the Protein Society · 2022Article
- Transcriptomics and Functional Analysis of Copper Stress Response in the Sulfate-Reducing BacteriumInternational journal of molecular sciences · 2022Article
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- Structural characterization of the microbial enzyme urocanate reductase mediating imidazole propionate production.Nature communications · 2021Article
- Article
- The effect of pH on Marinobacter hydrocarbonoclasticus denitrification pathway and nitrous oxide reductase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Impact of NaJournal of bacteriology · 2020Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Na(+)-translocating NADH:quinone oxidoreductase (Na(+)-NQR) contains two flavin residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. We demonstrate here that flavinylation of truncated Vibrio harveyi NqrC at Thr-229 in Escherichia coli cells requires the presence of a co-expressed Vibrio apbE gene. The apbE genes cluster with genes for Na(+)-NQR and other FMN-binding flavoproteins in bacterial genomes and encode proteins with previously unknown function. Experiments with isolated NqrC and ApbE proteins confirmed that ApbE is the only protein factor required for NqrC flavinylation and also indicated that the reaction is Mg(2+)-dependent and proceeds with FAD but not FMN. Inactivation of the apbE gene in Klebsiella pneumoniae, wherein the nqr operon and apbE are well separated in the chromosome, resulted in a complete loss of the quinone reductase activity of Na(+)-NQR, consistent with its dependence on covalently bound flavin. Our data thus identify ApbE as a novel modifying enzyme, flavin transferase.
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