ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
Regulation of ENaC in mice lacking renal insulin receptors in the collecting duct.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease.Purinergic signalling · 2026Article
- Epithelial NaComprehensive Physiology · 2024Review
- Sex Modulates Response to Renal-Tubule-Targeted Insulin Receptor Deletion in Mice.International journal of molecular sciences · 2023Article
- Aldosterone: Renal Action and Physiological Effects.Comprehensive Physiology · 2023Article
- Interleukin 6 mediated activation of the mineralocorticoid receptor in the aldosterone-sensitive distal nephron.American journal of physiology. Cell physiology · 2022Article
- Directing two-way traffic in the kidney: A tale of two ions.The Journal of general physiology · 2022Article
- Insulin Resistance and High Blood Pressure: Mechanistic Insight on the Role of the Kidney.Biomedicines · 2022Review
- Albumin Stimulates Epithelial NaInternational journal of molecular sciences · 2022Article
- "SLC-omics" of the kidney: solute transporters along the nephron.American journal of physiology. Cell physiology · 2021Review
- Hydrogen peroxide (HScientific reports · 2021Article
- Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel.Frontiers in physiology · 2021Article
- Salt-Sensitivity of Blood Pressure and Insulin Resistance.Frontiers in physiology · 2021Review
- Postprandial effects on electrolyte homeostasis in the kidney.American journal of physiology. Renal physiology · 2019Review
- Postprandial Effects on ENaC-Mediated Sodium Absorption.Scientific reports · 2019Article
- Ion channels and transporters in diabetic kidney disease.Current topics in membranes · 2019Review
- Role of connecting tubule glomerular feedback in obesity related renal damage.American journal of physiology. Renal physiology · 2018Article
- Renal tubule insulin receptor modestly promotes elevated blood pressure and markedly stimulates glucose reabsorption.JCI insight · 2018Article
- Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis.Kidney international · 2017Article
- The normal increase in insulin after a meal may be required to prevent postprandial renal sodium and volume losses.American journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Role and mechanisms of regulation of the basolateral KActa physiologica (Oxford, England) · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The epithelial sodium channel (ENaC) is one of the central effectors involved in regulation of salt and water homeostasis in the kidney. To study mechanisms of ENaC regulation, we generated knockout mice lacking the insulin receptor (InsR KO) specifically in the collecting duct principal cells. Single-channel analysis in freshly isolated split-open tubules demonstrated that the InsR-KO mice have significantly lower ENaC activity compared to their wild-type (C57BL/6J) littermates when animals were fed either normal or sodium-deficient diets. Immunohistochemical and Western blot assays demonstrated no significant changes in expression of ENaC subunits in InsR-KO mice compared to wild-type littermates. Insulin treatment caused greater ENaC activity in split-open tubules isolated from wild-type mice but did not have this effect in the InsR-KO mice. Thus, these results suggest that insulin increases ENaC activity via its own receptor affecting the channel open probability. To further determine the mechanism of the action of insulin on ENaC, we used mouse mpkCCDc14 principal cells. Insulin significantly augmented amiloride-sensitive transepithelial flux in these cells. Pretreatment of the mpkCCDc14 cells with phosphatidylinositol 3-kinase (LY294002; 10 μM) or mTOR (PP242; 100 nM) inhibitors precluded this effect. This study provides new information about the importance of insulin receptors expressed in collecting duct principal cells for ENaC activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.