ArticleImmunology letters2013
Sources of heterogeneity in human monocyte subsets.
Article in Immunology letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Association Between Monocyte Subsets and Cardiometabolic Disorders/Cardiovascular Disease: A Systematic Review and Meta-Analysis.Frontiers in cardiovascular medicine · 2021Pooled it
- A standardized single-tube 17-color spectral flow cytometry workflow for integrated immunophenotyping of human PBMCs and mixed co-culture systems.Frontiers in immunology · 2026Article
- Immune remodeling in chronic prostatitis: from microenvironment imbalance to targeted therapy.Frontiers in molecular biosciences · 2026Article
- Immunophenotyping of septic shock patients with endotheliopathy: focus on monocyte subtypes and Immune regulatory molecules.Frontiers in immunology · 2025Article
- Challenges of monocyte HLA-DR targeted immunomodulation in sepsis-a prospective observational cohort study.Frontiers in immunology · 2025Observational
- Human monocyte subtype expression of neuroinflammation- and regeneration-related genes is linked to age and sex.PloS one · 2024Article
- Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences.International journal of molecular sciences · 2023Review
- Alteration of monocyte subsets and their functions in thalassemia patients.International journal of hematology · 2023Article
- Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical Research.International journal of molecular sciences · 2022Article
- Montmorency Cherry Juice Consumption does not Improve Muscle Soreness or Inhibit Pro-inflammatory Monocyte Responses Following an Acute Bout of Whole-body Resistance Training.International journal of exercise science · 2022Article
- Article
- The frequency of interleukin-1β-producing monocytes is significantly associated with varicella-zoster responses of nursing home residents.Clinical and experimental immunology · 2021Article
- Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs.eLife · 2021Article
- Helminth species specific expansion and increased TNF-alpha production of non-classical monocytes during active tuberculosis.PLoS neglected tropical diseases · 2021Article
- Single-Cell and Bulk RNA-Sequencing Reveal Differences in Monocyte Susceptibility to Influenza A Virus Infection Between Africans and Europeans.Frontiers in immunology · 2021Article
- CSF1R defines the mononuclear phagocyte system lineage in human blood in health and COVID-19.Immunotherapy advances · 2021Article
- Monocyte Reconstitution and Gut Microbiota Composition after Hematopoietic Stem Cell Transplantation.Clinical hematology international · 2020Article
- Immune cell phenotyping in low blood volumes for assessment of cardiovascular disease risk, development, and progression: a pilot study.Journal of translational medicine · 2020Article
- Health Disparities in COVID-19: Addressing the Role of Social Determinants of Health in Immune System Dysfunction to Turn the Tide.Frontiers in public health · 2020Article
- Conventional Dendritic Cells and SlanFrontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Human monocytes are commonly defined and discriminated by the extent of their cell surface expression of CD14 and CD16, with associated differences in function and phenotype related to the intensity of expression of these markers. With increasing interest into the function and behaviour of monocytes, it is important to have a clear understanding of how differing strategies of analysis can affect results and how different protocols and population backgrounds can affect this highly morphogenic cell type. Using PBMCs from populations with differing ethnicities and histories of parasite exposure we have characterized monocyte phenotype based on intensity of CD14 and CD16 expression. Using the surface markers HLA-DR, CCR2 and CX3CR1, we compared monocyte phenotype between populations and further assessed changes in monocytes with freezing and thawing of PBMCs. Our results reveal that there is a progression of surface marker expression based on intensity of CD14 or CD16 expression, stressing the importance of careful gating of monocyte subtypes. Freezing and thawing of the PBMCs has no effect generally on the monocytes, although it does lead to a decrease in CD16 and CX3CR1 expression. We show that there are differences in the monocyte populations based on ethnicity and history of exposure to the common parasites Plasmodium falciparum and Schistosoma haematobium. This study highlights that blood monocytes consist of a continuous population of cells, within which the dominant phenotype may vary dependent on the background of the study population. Comparing results from monocyte studies therefore needs to be done with great care, as ethnic background of donor population, gating strategy and processing of PBMCs may all have an effect on outcome of monocyte phenotype.
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