ArticleParticle and fibre toxicology2013
Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface.
Article in Particle and fibre toxicology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 140 citations in OpenAlex.
- The potential adverse human health effects of metal-containing nanoparticles: a scoping review.Journal of occupational medicine and toxicology (London, England) · 2025Review
- Modular air-liquid interface aerosol exposure system (MALIES) to study toxicity of nanoparticle aerosols in 3D-cultured A549 cells in vitro.Archives of toxicology · 2024Article
- Nanoparticles: Taking a Unique Position in Medicine.Nanomaterials (Basel, Switzerland) · 2023Review
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture Models for Hazard Assessment of Nanomaterials.Nanomaterials (Basel, Switzerland) · 2023Article
- Liposomes or Extracellular Vesicles: A Comprehensive Comparison of Both Lipid Bilayer Vesicles for Pulmonary Drug Delivery.Polymers · 2023Review
- Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro.Nanomaterials (Basel, Switzerland) · 2022Article
- The Fate of Intranasally Instilled Silver Nanoarchitectures.Nano letters · 2022Article
- The Action-Networks of Nanosilver: Bridging the Gap between Material and Biology.Advanced healthcare materials · 2021Review
- Article
- Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.International journal of bioprinting · 2021Article
- Determining real-time mass deposition with a quartz crystal microbalance in an electrostatic, parallel-flow, air-liquid interface exposure system.Journal of aerosol science · 2021Article
- Parametric Optimization of an Air-Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeONanomaterials (Basel, Switzerland) · 2020Article
- Assessment of cytotoxicity and immune compatibility of phytochemicals-mediated biosynthesised silver nanoparticles usingIET nanobiotechnology · 2019Article
- In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires.Particle and fibre toxicology · 2019Article
- Silver nanoparticles as an effective disinfectant: A review.Materials science & engineering. C, Materials for biological applications · 2019Review
- In vitro detection of in vitro secondary mechanisms of genotoxicity induced by engineered nanomaterials.Particle and fibre toxicology · 2019Article
- Nanomaterial Exposure Induced Neutrophil Extracellular Traps: A New Target in Inflammation and Innate Immunity.Journal of immunology research · 2019Review
- Air-Liquid InterfaceApplied in vitro toxicology · 2018Review
- The impact of nanomaterial characteristics on inhalation toxicity.Toxicology research · 2018Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDue to its antibacterial properties, silver (Ag) has been used in more consumer products than any other nanomaterial so far. Despite the promising advantages posed by using Ag-nanoparticles (NPs), their interaction with mammalian systems is currently not fully understood. An exposure route via inhalation is of primary concern for humans in an occupational setting. Aim of this study was therefore to investigate the potential adverse effects of aerosolised Ag-NPs using a human epithelial airway barrier model composed of A549, monocyte derived macrophage and dendritic cells cultured in vitro at the air-liquid interface. Cell cultures were exposed to 20 nm citrate-coated Ag-NPs with a deposition of 30 and 278 ng/cm2 respectively and incubated for 4 h and 24 h. To elucidate whether any effects of Ag-NPs are due to ionic effects, Ag-Nitrate (AgNO3) solutions were aerosolised at the same molecular mass concentrations.
resultsAgglomerates of Ag-NPs were detected at 24 h post exposure in vesicular structures inside cells but the cellular integrity was not impaired upon Ag-NP exposures. Minimal cytotoxicity, by measuring the release of lactate dehydrogenase, could only be detected following a higher concentrated AgNO3-solution. A release of pro-inflammatory markers TNF-α and IL-8 was neither observed upon Ag-NP and AgNO3 exposures as well as was not affected when cells were pre-stimulated with lipopolysaccharide (LPS). Also, an induction of mRNA expression of TNF-α and IL-8, could only be observed for the highest AgNO3 concentration alone or even significantly increased when pre-stimulated with LPS after 4 h. However, this effect disappeared after 24 h. Furthermore, oxidative stress markers (HMOX-1, SOD-1) were expressed after 4 h in a concentration dependent manner following AgNO3 exposures only.
conclusionsWith an experimental setup reflecting physiological exposure conditions in the human lung more realistic, the present study indicates that Ag-NPs do not cause adverse effects and cells were only sensitive to high Ag-ion concentrations. Chronic exposure scenarios however, are needed to reveal further insight into the fate of Ag-NPs after deposition and cell interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.