ArticleTherapeutic delivery2013
Cholesterol domains enhance transfection.
Article in Therapeutic delivery, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Lipid-based nucleic acid therapeutics with in vivo efficacy.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2023Review
- The Effect of Repeat Administration of Lipoplexes on Gene Delivery, Biodistribution, and Cytokine Response in Immunocompetent Tumor-Bearing Mice.Journal of pharmaceutical sciences · 2022Article
- Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- A novel method for conjugating the terminal amine of peptide ligands to cholesterol: synthesis iRGD-cholesterol.Therapeutic delivery · 2019Article
- Nanoparticle uptake by circulating leukocytes: A major barrier to tumor delivery.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Relating toxicity to transfection: using sphingosine to maintain prolonged expression in vitro.Molecular pharmaceutics · 2015Article
- Gene delivery to Her-2+ breast cancer cells using a two-component delivery system to achieve specificity.Nanomedicine : nanotechnology, biology, and medicine · 2014Article
- Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: interplay between nanostructure and composition.Biochimica et biophysica acta · 2014Article
- The effects of lipoplex formulation variables on the protein corona and comparisons with in vitro transfection efficiency.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Gene transfection in high serum levels: case studies with new cholesterol based cationic gemini lipids.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundThe formation of cholesterol domains in lipoplexes has been associated with enhanced serum stability and transfection rates both in cell culture and in vivo.
resultsThis study utilizes the ability of saturated phosphatidylcholines to promote the formation of cholesterol domains at much lower cholesterol contents than have been utilized in previous work. The results demonstrate that lipoplexes with identical cholesterol and cationic lipid contents exhibit significantly improved transfection efficiencies when a domain is present, consistent with previous work. In addition, studies assessing transfection rates in the absence of serum demonstrate that the ability of domains to enhance transfection is not dependent on interactions with serum proteins. Consistent with this hypothesis, characterization of the adsorbed proteins composing the corona of these lipoplex formulations did not reveal a correlation between transfection and the adsorption of a specific protein.
conclusionWe have demonstrated that the interaction with serum proteins can promote domain formation in some formulations, and thereby result in enhanced transfection only after serum exposure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.