ArticleParasites & vectors2013
SvSXP: a Strongylus vulgaris antigen with potential for prepatent diagnosis.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 54 citations in OpenAlex.
- Diagnostic innovations in Equine Parasitology: a Nanogold-ELISA for sensitive serodiagnosis of migratory strongylus vulgaris larvae infections.BMC veterinary research · 2024Article
- A retrospective study of the prevalence in equine postmortems of cranial mesenteric arteritis caused byThe Canadian veterinary journal = La revue veterinaire canadienne · 2024Article
- The Use of Innovative Diagnostics to Inform Sustainable Control of Equine Helminth Infections.Pathogens (Basel, Switzerland) · 2023Review
- Morphomolecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Parasite dynamics in untreated horses through one calendar year.Parasites & vectors · 2022Article
- Occurrence of Strongylid Nematode Parasites on Horse Farms in Berlin and Brandenburg, Germany, With High Seroprevalence ofFrontiers in veterinary science · 2022Article
- Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients.Animals : an open access journal from MDPI · 2020Article
- Parasite Occurrence and Parasite Management in Swedish Horses Presenting with Gastrointestinal Disease-A Case-Control Study.Animals : an open access journal from MDPI · 2020Article
- Idiopathic peritonitis in horses: a retrospective study of 130 cases in Sweden (2002-2017).Acta veterinaria Scandinavica · 2019Article
- Th1/Th2 balance and humoral immune response to potential antigens as early diagnostic method of equineVeterinary world · 2017Article
- Detection of Strongylus vulgaris in equine faecal samples by real-time PCR and larval culture - method comparison and occurrence assessment.BMC veterinary research · 2017Article
- Serum Strongylus vulgaris-specific antibody responses to anthelmintic treatment in naturally infected horses.Parasitology research · 2015Article
- Changes in Serum Strongylus Vulgaris-Specific Antibody Concentrations in Response to Anthelmintic Treatment of Experimentally Infected Foals.Frontiers in veterinary science · 2015Article
- A survey on parasite management by equine veterinarians highlights the need for a regulation change.Veterinary record open · 2015Article
- Anthelmintic resistance in equine nematodes.International journal for parasitology. Drugs and drug resistance · 2014Review
- In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products.Parasitology research · 2014Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStrongyle parasites are ubiquitous in grazing horses. Strongylus vulgaris, the most pathogenic of the large strongyles, is known for its extensive migration in the mesenteric arterial system. The lifecycle of S. vulgaris is characterised by a long prepatent period where the migrating larvae are virtually undetectable as there currently is no test available for diagnosing prepatent S. vulgaris infection. Presence of S. vulgaris larvae in the arterial system causes endarteritis and thrombosis with a risk of non-strangulating intestinal infarctions. Emergence of anthelmintic resistance among cyathostomins has led to recommendations of reduced treatment intensity by targeting horses that exceed a predetermined strongyle faecal egg count threshold. One study suggests an apparent increase in prevalence of S. vulgaris on farms where reduced anthelmintic treatment intensity has been implemented. These issues highlight the need for an accurate and reliable assay for diagnosing prepatent S. vulgaris infection.
methodsImmunoscreening of a larval S. vulgaris cDNA library using hyperimmune serum raised against S. vulgaris excretory/secretory antigens was performed to identify potential diagnostic antigens. Immunoreactive clones were sequenced, one potential antigen was characterised, expressed as a recombinant protein, initially evaluated by western blot (WB) analysis, the diagnostic potential of the IgG subclasses was evaluated by ELISA, and the diagnostic accuracy evaluated using serum from 102 horses with known S. vulgaris infection status.
resultsThe clone expressing the potential antigen encoded a S. vulgaris SXP/RAL2 homologue. The recombinant protein, rSvSXP, was shown to be a potential diagnostic antigen by WB analysis, and a target of serum IgGa, IgG(T) and total IgG in naturally infected horses, with IgG(T) antibodies being the most reliable indicator of S. vulgaris infection in horses. Evaluation of diagnostic accuracy of the ELISA resulted in a sensitivity of 73.3%, a specificity of 81.0%, a diagnostic odds ratio of 11.69; a positive likelihood ratio (LR) of 3.85 and a negative LR was 0.33. The area under the ROC curve was 0.820.
conclusionIgG(T) antibodies to recombinant SvSXP show potential for use as an antigen for prepatent diagnosis of migrating stages of S. vulgaris with moderate to good diagnostic accuracy.
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