ArticleTissue engineering. Part C, Methods2013
Designing a multicellular organotypic 3D liver model with a detachable, nanoscale polymeric Space of Disse.
Article in Tissue engineering. Part C, Methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Comparative transcriptomic and phenotypic analysis of induced pluripotent stem cell hepatocyte-like cells and primary human hepatocytes.Cell and tissue research · 2024Article
- Recent Developments in Layer-by-Layer Assembly for Drug Delivery and Tissue Engineering Applications.Advanced healthcare materials · 2024Review
- Exploiting maleimide-functionalized hyaluronan hydrogels to test cellular responses to physical and biochemical stimuli.Biomedical materials (Bristol, England) · 2022Article
- Matrix stiffness and shear stresses modulate hepatocyte functions in a fibrotic liver sinusoidal model.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Current progress in hepatic tissue regeneration by tissue engineering.Journal of translational medicine · 2019Review
- Advances in Engineered Human Liver Platforms for Drug Metabolism Studies.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- Transcriptomic Analysis of Hepatic Cells in Multicellular Organotypic Liver Models.Scientific reports · 2018Article
- Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies.Cellular and molecular gastroenterology and hepatology · 2018Review
- Engineering Cell Surfaces with Polyelectrolyte Materials for Translational Applications.Polymers · 2017Review
- The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment.Proteome science · 2016Article
- Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization.Journal of visualized experiments : JoVE · 2015Article
- Substrate stiffness regulates primary hepatocyte functions.RSC advances · 2015Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
The design of in vitro models that mimic the stratified multicellular hepatic microenvironment continues to be challenging. Although several in vitro hepatic cultures have been shown to exhibit liver functions, their physiological relevance is limited due to significant deviation from in vivo cellular composition. We report the assembly of a novel three-dimensional (3D) organotypic liver model incorporating three different cell types (hepatocytes, liver sinusoidal endothelial cells, and Kupffer cells) and a polymeric interface that mimics the Space of Disse. The nanoscale interface is detachable, optically transparent, derived from self-assembled polyelectrolyte multilayers, and exhibits a Young's modulus similar to in vivo values for liver tissue. Only the 3D liver models simultaneously maintain hepatic phenotype and elicit proliferation, while achieving cellular ratios found in vivo. The nanoscale detachable polymeric interfaces can be modulated to mimic basement membranes that exhibit a wide range of physical properties. This facile approach offers a versatile new avenue in the assembly of engineered tissues. These results demonstrate the ability of the tri-cellular 3D cultures to serve as an organotypic hepatic model that elicits proliferation and maintenance of phenotype and in vivo-like cellular ratios.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.