ArticlePloS one2013
Site specific mutation of the Zic2 locus by microinjection of TALEN mRNA in mouse CD1, C3H and C57BL/6J oocytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Mutagenesis on a complex mouse genetic background by site-specific nucleases.Transgenic research · 2024Article
- Mechanistic toxicology in light of genetic compensation.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Review
- Genome engineering and disease modelingWorld journal of stem cells · 2021Review
- Genome-wide CRISPR screen identifies ZIC2 as an essential gene that controls the cell fate of early mesodermal precursors to human heart progenitors.Stem cells (Dayton, Ohio) · 2020Article
- Principles of Genetic Engineering.Genes · 2020Review
- A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity.Scientific reports · 2018Article
- Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo.Nature cell biology · 2017Article
- Subfunctionalization of Paralogous Aryl Hydrocarbon Receptors from the Frog Xenopus Laevis: Distinct Target Genes and Differential Responses to Specific Agonists in a Single Cell Type.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer.Scientific reports · 2016Article
- Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases.Asian-Australasian journal of animal sciences · 2016Article
- TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis.Stem cells and development · 2015Article
- TALEN-mediated genome engineering to generate targeted mice.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- mRNA-based therapeutics--developing a new class of drugs.Nature reviews. Drug discovery · 2014Review
- Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems.Scientific reports · 2014Article
- TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery.Acta naturae · 2014Article
- Review
- Simultaneous gene editing by injection of mRNAs encoding transcription activator-like effector nucleases into mouse zygotes.Molecular and cellular biology · 2014Article
- TALENs mediate efficient and heritable mutation of endogenous genes in the marine annelid Platynereis dumerilii.Genetics · 2014Article
- Genetically engineered humanized mouse models for preclinical antibody studies.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Transcription Activator-Like Effector Nucleases (TALENs) consist of a nuclease domain fused to a DNA binding domain which is engineered to bind to any genomic sequence. These chimeric enzymes can be used to introduce a double strand break at a specific genomic site which then can become the substrate for error-prone non-homologous end joining (NHEJ), generating mutations at the site of cleavage. In this report we investigate the feasibility of achieving targeted mutagenesis by microinjection of TALEN mRNA within the mouse oocyte. We achieved high rates of mutagenesis of the mouse Zic2 gene in all backgrounds examined including outbred CD1 and inbred C3H and C57BL/6J. Founder mutant Zic2 mice (eight independent alleles, with frameshift and deletion mutations) were created in C3H and C57BL/6J backgrounds. These mice transmitted the mutant alleles to the progeny with 100% efficiency, allowing the creation of inbred lines. Mutant mice display a curly tail phenotype consistent with Zic2 loss-of-function. The efficiency of site-specific germline mutation in the mouse confirm TALEN mediated mutagenesis in the oocyte to be a viable alternative to conventional gene targeting in embryonic stem cells where simple loss-of-function alleles are required. This technology enables allelic series of mutations to be generated quickly and efficiently in diverse genetic backgrounds and will be a valuable approach to rapidly create mutations in mice already bearing one or more mutant alleles at other genetic loci without the need for lengthy backcrossing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.