ArticlePloS one2013
Low cost extraction and isothermal amplification of DNA for infectious diarrhea diagnosis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 70 citations in OpenAlex.
- Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics.Biosensors · 2024Review
- Article
- An integrated isothermal nucleic acid amplification test to detect HPV16 and HPV18 DNA in resource-limited settings.Science translational medicine · 2023Article
- Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples.Sensors (Basel, Switzerland) · 2021Review
- Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings.Analytical chemistry · 2021Article
- Rapid electrostatic DNA enrichment for sensitive detection ofAnalytical methods : advancing methods and applications · 2020Article
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Amplification chemistries in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2019Review
- Pressure-Modulated Selective Electrokinetic Trapping for Direct Enrichment, Purification, and Detection of Nucleic Acids in Human Serum.Analytical chemistry · 2018Article
- Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.Analytical biochemistry · 2018Article
- New Molecular Diagnostic Approaches to Bacterial Infections and Antibacterial Resistance.Annual review of medicine · 2018Review
- Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection.Analytical and bioanalytical chemistry · 2018Review
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters.Environmental science & technology · 2017Article
- Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection.Frontiers in plant science · 2017Review
- Future developments in biosensors for field-ready Zika virus diagnostics.Journal of biological engineering · 2017Review
- A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples.Lab on a chip · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
In order to counter the common perception that molecular diagnostics are too complicated to work in low resource settings, we have performed a difficult sample preparation and DNA amplification protocol using instrumentation designed to be operated without wall or battery power. In this work we have combined a nearly electricity-free nucleic acid extraction process with an electricity-free isothermal amplification assay to detect the presence of Clostridium difficile (C. difficile) DNA in the stool of infected patients. We used helicase-dependent isothermal amplification (HDA) to amplify the DNA in a low-cost, thermoplastic reaction chip heated with a pair of commercially available toe warmers, while using a simple Styrofoam insulator. DNA was extracted from known positive and negative stool samples. The DNA extraction protocol utilized an air pressure driven solid phase extraction device run using a standard bicycle pump. The simple heater setup required no electricity or battery and was capable of maintaining the temperature at 65°C±2°C for 55 min, suitable for repeatable HDA amplification. Experiments were performed to explore the adaptability of the system for use in a range of ambient conditions. When compared to a traditional centrifuge extraction protocol and a laboratory thermocycler, this disposable, no power platform achieved approximately the same lower limit of detection (1.25×10(-2) pg of C. difficile DNA) while requiring much less raw material and a fraction of the lab infrastructure and cost. This proof of concept study could greatly impact the accessibility of molecular assays for applications in global health.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.