ArticlePloS one2013
Pyrosequencing reveals high-temperature cellulolytic microbial consortia in Great Boiling Spring after in situ lignocellulose enrichment.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
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- Biochemical characterization of Fsa16295Glu fromFrontiers in microbiology · 2024Article
- Mcr-dependent methanogenesis in Archaeoglobaceae enriched from a terrestrial hot spring.The ISME journal · 2023Article
- An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea.Nature communications · 2022Article
- Thermophiles and carbohydrate-active enzymes (CAZymes) in biofilm microbial consortia that decompose lignocellulosic plant litters at high temperatures.Scientific reports · 2022Article
- Bacterial community structure in geothermal springs on the northern edge of Qinghai-Tibet plateau.Frontiers in microbiology · 2022Article
- High Potential for Biomass-Degrading Enzymes Revealed by Hot Spring Metagenomics.Frontiers in microbiology · 2021Article
- Genomics, Exometabolomics, and Metabolic Probing Reveal Conserved Proteolytic Metabolism ofFrontiers in microbiology · 2021Article
- Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere.The ISME journal · 2020Article
- Novel Hyperthermophilic CrenarchaeonFrontiers in microbiology · 2019Article
- Enrichment and Characterisation of a Mixed-Source Ethanologenic Community Degrading the Organic Fraction of Municipal Solid Waste Under Minimal Environmental Control.Frontiers in microbiology · 2019Article
- Microbial diversity of thermophiles with biomass deconstruction potential in a foliage-rich hot spring.MicrobiologyOpen · 2018Article
- Succession of lignocellulolytic bacterial consortia bred anaerobically from lake sediment.Microbial biotechnology · 2016Article
- Phylogeny and physiology of candidate phylum 'Atribacteria' (OP9/JS1) inferred from cultivation-independent genomics.The ISME journal · 2016Article
- High-Quality Draft Genome Sequence of Kallotenue papyrolyticum JKG1T Reveals Broad Heterotrophic Capacity Focused on Carbohydrate and Amino Acid Metabolism.Genome announcements · 2015Article
- Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments.The ISME journal · 2015Article
- Microbial community composition and ultrastructure of granules from a full-scale anammox reactor.Microbial ecology · 2015Article
- Community analysis of plant biomass-degrading microorganisms from Obsidian Pool, Yellowstone National Park.Microbial ecology · 2015Article
- Microbial Communities and Organic Matter Composition in Surface and Subsurface Sediments of the Helgoland Mud Area, North Sea.Frontiers in microbiology · 2015Article
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Abstract
To characterize high-temperature cellulolytic microbial communities, two lignocellulosic substrates, ammonia fiber-explosion-treated corn stover and aspen shavings, were incubated at average temperatures of 77 and 85°C in the sediment and water column of Great Boiling Spring, Nevada. Comparison of 109,941 quality-filtered 16S rRNA gene pyrosequences (pyrotags) from eight enrichments to 37,057 quality-filtered pyrotags from corresponding natural samples revealed distinct enriched communities dominated by phylotypes related to cellulolytic and hemicellulolytic Thermotoga and Dictyoglomus, cellulolytic and sugar-fermenting Desulfurococcales, and sugar-fermenting and hydrogenotrophic Archaeoglobales. Minor enriched populations included close relatives of hydrogenotrophic Thermodesulfobacteria, the candidate bacterial phylum OP9, and candidate archaeal groups C2 and DHVE3. Enrichment temperature was the major factor influencing community composition, with a negative correlation between temperature and richness, followed by lignocellulosic substrate composition. This study establishes the importance of these groups in the natural degradation of lignocellulose at high temperatures and suggests that a substantial portion of the diversity of thermophiles contributing to consortial cellulolysis may be contained within lineages that have representatives in pure culture.
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