ArticlePloS one2013
Oxidative stress induces monocyte necrosis with enrichment of cell-bound albumin and overexpression of endoplasmic reticulum and mitochondrial chaperones.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
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- MicroRNAs in IgA nephropathy.Renal failure · 2021Review
- Crosstalk between endoplasmic reticulum stress and oxidative stress in the progression of diabetic nephropathy.Cell stress & chaperones · 2021Article
- Association of temperament with genetic polymorphisms inMolecular biology research communications · 2021Article
- Proteomics-based analysis indicating α-enolase as a potential biomarker in primary Sjögren's syndrome.Gland surgery · 2020Article
- Two cancer stem cell-targeted therapies in clinical trials as viewed from the standpoint of the cancer stem cell model.Stem cells translational medicine · 2020Article
- Curcumin Affects HSP60 Folding Activity and Levels in Neuroblastoma Cells.International journal of molecular sciences · 2020Article
- Hsp60 Post-translational Modifications: Functional and Pathological Consequences.Frontiers in molecular biosciences · 2020Review
- Review
- A genetic variant in SLC30A2 causes breast dysfunction during lactation by inducing ER stress, oxidative stress and epithelial barrier defects.Scientific reports · 2018Article
- miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1.Oxidative medicine and cellular longevity · 2017Article
- Methyl methanesulfonate induces necroptosis in human lung adenoma A549 cells through the PIG-3-reactive oxygen species pathway.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Proinsulin and heat shock protein 90 as biomarkers of beta-cell stress in the early period after onset of type 1 diabetes.Translational research : the journal of laboratory and clinical medicine · 2016Article
- Effects of 5'-azacytidine and alendronate on a hepatocellular carcinoma cell line: a proteomics perspective.Molecular and cellular biochemistry · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, monocytes were treated with 5-azacytidine (azacytidine), gossypol or hydrogen peroxide to induce cell death through oxidative stress. A shift from apoptotic to necrotic cell death occurred when monocytes were treated with 100 µM azacytidine for more than 12 hours. Necrotic monocytes exhibited characteristics, including enrichment of cell-bound albumin and up-regulation of endoplasmic reticulum (ER)- and mitochondrial-specific chaperones to protect mitochondrial integrity, which were not observed in other necrotic cells, including HUH-7, A2780, A549 and HOC1a. Our results show that the cell-bound albumin originates in the culture medium rather than from monocyte-derived hepatocytes, and that HSP60 is a potential binding partner of the cell-bound albumin. Proteomic analysis shows that HSP60 and protein disulfide isomerase are the most abundant up-regulated mitochondrial and ER-chaperones, and that both HSP60 and calreticulin are ubiquitinated in necrotic monocytes. In contrast, expression levels of the cytosolic chaperones HSP90 and HSP71 were down-regulated in the azacytidine-treated monocytes, concomitant with an increase in the levels of these chaperones in the cell culture medium. Collectively, our results demonstrates that chaperones from different organelles behave differently in necrotic monocytes, ER- and mitochondrial chaperones being retained and cytosolic and nuclear chaperones being released into the cell culture medium through the ruptured cell membrane. HSP60 may serve as a new target for development of myeloid leukemia treatment.
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