ArticlePloS one2013
Automated transmission-mode scanning electron microscopy (tSEM) for large volume analysis at nanoscale resolution.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 74 citations in OpenAlex.
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- PyReconstruct: A fully open-source, collaborative successor to Reconstruct.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ultrastructural Contributions to Extrasynaptic Glutamatergic Signaling in Olfactory Bulb Glomeruli.The Journal of comparative neurology · 2025Article
- Long-Term Potentiation Produces a Sustained Expansion of Synaptic Information Storage Capacity in Adult Rat Hippocampus.bioRxiv : the preprint server for biology · 2024Article
- Neurons with larval synaptic targets pioneer the later nervous system in the annelidFrontiers in neuroscience · 2024Article
- Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
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- Dendritic Spine Density Scales with Microtubule Number in Rat Hippocampal Dendrites.Neuroscience · 2022Article
- Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies.Animals : an open access journal from MDPI · 2021Review
- Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Deep learning-based point-scanning super-resolution imaging.Nature methods · 2021Article
- The visual pigment xenopsin is widespread in protostome eyes and impacts the view on eye evolution.eLife · 2020Article
- Application of automated electron microscopy imaging and machine learning to characterise and quantify nanoparticle dispersion in aqueous media.Journal of microscopy · 2020Article
- Synaptic Odyssey.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation.PloS one · 2020Article
- Editorial: Electron-Microscopy-Based Tools for Imaging Cellular Circuits and Organisms.Frontiers in neural circuits · 2019Article
- A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure.Nature communications · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transmission-mode scanning electron microscopy (tSEM) on a field emission SEM platform was developed for efficient and cost-effective imaging of circuit-scale volumes from brain at nanoscale resolution. Image area was maximized while optimizing the resolution and dynamic range necessary for discriminating key subcellular structures, such as small axonal, dendritic and glial processes, synapses, smooth endoplasmic reticulum, vesicles, microtubules, polyribosomes, and endosomes which are critical for neuronal function. Individual image fields from the tSEM system were up to 4,295 µm(2) (65.54 µm per side) at 2 nm pixel size, contrasting with image fields from a modern transmission electron microscope (TEM) system, which were only 66.59 µm(2) (8.160 µm per side) at the same pixel size. The tSEM produced outstanding images and had reduced distortion and drift relative to TEM. Automated stage and scan control in tSEM easily provided unattended serial section imaging and montaging. Lens and scan properties on both TEM and SEM platforms revealed no significant nonlinear distortions within a central field of ∼100 µm(2) and produced near-perfect image registration across serial sections using the computational elastic alignment tool in Fiji/TrakEM2 software, and reliable geometric measurements from RECONSTRUCT™ or Fiji/TrakEM2 software. Axial resolution limits the analysis of small structures contained within a section (∼45 nm). Since this new tSEM is non-destructive, objects within a section can be explored at finer axial resolution in TEM tomography with current methods. Future development of tSEM tomography promises thinner axial resolution producing nearly isotropic voxels and should provide within-section analyses of structures without changing platforms. Brain was the test system given our interest in synaptic connectivity and plasticity; however, the new tSEM system is readily applicable to other biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.