ArticlePloS one2013
RNAi-mediated knockdown of catalase causes cell cycle arrest in SL-1 cells and results in low survival rate of Spodoptera litura (Fabricius).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Identification and characterization of catalase genes involved in the response to heat stress in Tetranychus urticae (Acari: Tetranychidae).BMC genomics · 2025Article
- Microsporidian infection of mosquito larvae changes the host-associated microbiome towards the synthesis of antimicrobial factors.Parasites & vectors · 2025Article
- Extensive influence of microsporidian infection on sucrose solution consumption, antioxidant enzyme activity, cell structure, and lifespan of Asian honeybees.Frontiers in immunology · 2024Article
- A Review on Digestive System ofInsects · 2023Review
- A simple method for ex vivo honey bee cell culture capable of in vitro gene expression analysis.PloS one · 2021Article
- Characterization of cell lines derived from the southern armyworm, Spodoptera eridania.In vitro cellular & developmental biology. Animal · 2018Article
- Influence of UV-A radiation on oxidative stress and antioxidant enzymes in Mythimna separata (Lepidoptera: Noctuidae).Environmental science and pollution research international · 2017Article
- Review
- Quantitative RT-PCR Gene Evaluation and RNA Interference in the Brown Marmorated Stink Bug.PloS one · 2016Article
- Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing.Frontiers in physiology · 2015Review
- Effects of Pyriproxyfen on Female Reproduction in the Common Cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae).PloS one · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deregulated reactive oxygen species (ROS) production can lead to the disruption of structural and functional integrity of cells as a consequence of reactive interaction between ROS and various biological components. Catalase (CAT) is a common enzyme existing in nearly all organisms exposed to oxygen, which decomposes harmful hydrogen peroxide, into water and oxygen. In this study, the full length sequence that encodes CAT-like protein from Spodoptera litura named siltCAT (GenBank accession number: JQ_663444) was cloned and characterized. Amino acid sequence alignment showed siltCAT shared relatively high conservation with other insect, especially the conserved residues which defined heme and NADPH orientation. Expression pattern analysis showed that siltCAT mRNA was mainly expressed in the fat body, midgut, cuticle and malpighian tube, and as well as over last instar larvae, pupa and adult stages. RNA interference was used to silence CAT gene in SL-1 cells and the fourth-instar stage of S. litura larvae respectively. Our results provided evidence that CAT knockdown induced ROS generation, cell cycle arrest and apoptosis in SL-1 cells. It also confirmed the decrease in survival rate because of increased ROS production in experimental groups injected with double-stranded RNA of CAT (dsCAT). This study implied that ROS scavenging by CAT is important for S. litura survival.
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