ArticleWorld journal of gastroenterology2013
Evaluation of the relationship between dietary factors, CagA-positive Helicobacter pylori infection, and RUNX3 promoter hypermethylation in gastric cancer tissue.
Article in World journal of gastroenterology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 22 citations in OpenAlex.
- Runt-related transcription factors: from pathogenesis to therapeutic targets in multiple-organ fibrosis.Frontiers in cell and developmental biology · 2025Review
- How Dietary Factors Affect DNA Methylation: Lesson from Epidemiological Studies.Medicina (Kaunas, Lithuania) · 2020Review
- Structure of cytotoxic associated antigen A protein ofVeterinary world · 2020Article
- Risk factors for gastric precancerous and cancers lesions in Latin American counties with difference gastric cancer risk.Cancer epidemiology · 2020Article
- DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort.Epigenetics · 2019Article
- Mislocalization of Runt-related transcription factor 3 results in airway inflammation and airway hyper-responsiveness in a murine asthma model.Experimental and therapeutic medicine · 2017Article
- Epigenetic changes in localized gastric cancer: the role of RUNX3 in tumor progression and the immune microenvironment.Oncotarget · 2016Article
- Multiplex H. pylori Serology and Risk of Gastric Cardia and Noncardia Adenocarcinomas.Cancer research · 2015Article
- Challenges of deciphering gastric cancer heterogeneity.World journal of gastroenterology · 2015Review
- MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB.Molecular medicine reports · 2015Article
- Helicobacter pylori virulence factor CagA promotes tumorigenesis of gastric cancer via multiple signaling pathways.Cell communication and signaling : CCS · 2015Review
- Helicobacter pylori: Genomic Insight into the Host-Pathogen Interaction.International journal of genomics · 2015Review
- Seroepidemiology of Helicobacter pylori infection in elderly people in the Beijing region, China.World journal of gastroenterology · 2014Article
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Authors and funding
10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the relationship among Helicobacter pylori (H. pylori) infection, CagA status, and dietary factors with RUNX3 promoter hypermethylation.
methodsGastric cancer tissue samples were collected from 184 South Korean patients. All patients were interviewed following a semi-quantitative food frequency questionnaire. The average frequencies of intake and portion sizes of 89 common food items were documented, and total intakes of calories, nutrients, vitamins, and minerals were calculated for each subject. DNA was extracted from gastric cancer tissue samples, and amplification of the HSP60 gene was performed to detect H. pylori infection. Nested polymerase chain reaction (PCR) was used to detect the presence of the CagA gene. RUNX3 gene expression was measured by reverse transcription-PCR, and RUNX3 methylation status was evaluated by methylation-specific PCR. The odds ratios (ORs) and 95%CI associated with RUNX3 promoter hypermethylation status were estimated for each of the food groups, lifestyle factors, and the interaction between dietary and lifestyle factors with CagA status of H. pylori infection.
resultsOverall, 164 patients (89.1%) were positive for H. pylori DNA, with the CagA gene detected in 59 (36%) of these H. pylori-positive samples. In all, 106 (57.6%) patients with gastric cancer demonstrated CpG island hypermethylation at the RUNX3 promoter. RUNX3 expression was undetectable in 52 (43.7%) of the 119 gastric cancer tissues sampled. A high consumption of eggs may increase the risk of RUNX3 methylation in gastric cancer patients, having a mean OR of 2.15 (range, 1.14-4.08). A significantly increased OR of 4.28 (range, 1.19-15.49) was observed with a high consumption of nuts in patients with CagA-positive H. pylori infection. High intakes of carbohydrate, vitamin B1, and vitamin E may decrease the risk of RUNX3 methylation in gastric cancer tissue, particularly in CagA- or H. pylori-negative infection, with OR of 0.41 (0.19-0.90), 0.42 (0.20-0.89), and 0.29 (0.13-0.62), respectively. A high consumption of fruits may protect against RUNX3 methylation.
conclusionThese results suggest that the CagA status of H. pylori infection may be a modifier of dietary effects on RUNX3 methylation in gastric cancer tissue.
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