ArticleJournal of biomedical research2012
Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis.
Article in Journal of biomedical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Sperm Membrane Stability: In-Depth Analysis from Structural Basis to Functional Regulation.Veterinary sciences · 2025Review
- The ontogenic gonadal transcriptomes provide insights into sex change in the ricefield eel Monopterus albus.BMC zoology · 2022Article
- The control of male fertility by spermatid-specific factors: searching for contraceptive targets from spermatozoon's head to tail.Cell death & disease · 2016Review
- Article
- TDRP deficiency contributes to low sperm motility and is a potential risk factor for male infertility.American journal of translational research · 2016Article
- Expression of Flotilin-2 and Acrosome Biogenesis Are Regulated by MiR-124 during Spermatogenesis.PloS one · 2015Article
- Proteogenomics of Gammarus fossarum to document the reproductive system of amphipods.Molecular & cellular proteomics : MCP · 2014Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SPERMATOGENESIS IS A COMPLEX PROCESS OF TERMINAL DIFFERENTIATION BY WHICH MATURE SPERMS ARE GENERATED, AND IT CAN BE DIVIDED INTO THREE PHASES: mitosis, meiosis and spermiogenesis. In a previous study, we established a series of proteomic profiles for spermatogenesis to understand the regulation of male fertility and infertility. Here, we further investigated the localization and the role of flotillin-2 in spermiogenesis. Flotillin-2 expression was investigated in the testis of male CD1 mice at various developmental stages of spermatogenesis by using Western blotting, immunohistochemistry and immunofluorescence. Flotillin-2 was knocked down in vivo in three-week-old male mice using intratesticular injection of small inhibitory RNA (siRNA), and sperm abnormalities were assessed three weeks later. Flotillin-2 was expressed at high levels in male germ cells during spermatogenesis. Flotillin-2 immunoreactivity was observed in pachytene spermatocytes as a strong dot-shaped signal and in round spermatids as a sickle-shaped distribution ahead of the acrosome. Immunofluorescence confirmed flotillin-2 was localized in front of the acrosome in round spermatids, indicating that flotillin-2 was localized to the Golgi apparatus. Knockdown of flotillin-2 in vivo led to a significant increase in head sperm abnormalities isolated from the cauda epididymis, compared with control siRNA-injected testes. This study indicates that flotillin-2 is a novel Golgi-related protein involved in sperm acrosome biogenesis.
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