Evidence map›Paper›PMID 23554189›Full record

ArticleJournal of clinical microbiology2013

Fifty percent tissue culture infective dose assay for determining the titer of infectious human herpesvirus 8.

Sagar V Nadgir, Heather R Hensler, Emilee R Knowlton, Charles R Rinaldo, Giovanna Rappocciolo, Frank J Jenkins

Abstract readValidation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sagar V NadgirDepartment of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Heather R Hensler
Emilee R Knowlton
Charles R Rinaldo
Giovanna Rappocciolo
Frank J Jenkins

Funding

University of Pittsburgh Multicenter AIDS Cohort Study (MACS)U01AI035041 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MARTINSON, JEREMY JAMES, RINALDO, CHARLES R · 1993 to 2018
$71.4M
CYTOTOXIC T CELL RESPONSES TO HHV8R01CA082053 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI RINALDO, CHARLES R · 1999 to 2013
$5.2M
NCI NIH HHS CA082053NCI NIH HHS R01 CA082053NIAID NIH HHS U01 AI035041NIAID NIH HHS U01AI035041
6 · The paper itself

Abstract

We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID50 levels. Validation of TCID50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID50 doses.

Indexed as

beta-GalactosidaseCell LineDendritic CellsGenes, ReporterHerpesvirus 8, HumanHumansViral Loadbeta-Galactosidase

Identifiers

PMID23554189
PMCPMC3716109

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.