ArticleEMBO molecular medicine2013
Actin filament dynamics impacts keratinocyte stem cell maintenance.
Article in EMBO molecular medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Article
- Ontogeny of Skin Stem Cells and Molecular Underpinnings.Current issues in molecular biology · 2024Review
- Biochemical role of FOXM1-dependent histone linker H1B in human epidermal stem cells.Cell death & disease · 2024Article
- Article
- The EGF/EGFR axis and its downstream signaling pathways regulate the motility and proliferation of cultured oral keratinocytes.FEBS open bio · 2023Article
- Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures.EMBO reports · 2023Article
- Stairways to Advanced Therapies for Epidermolysis Bullosa.Cold Spring Harbor perspectives in biology · 2023Review
- pTINCR microprotein promotes epithelial differentiation and suppresses tumor growth through CDC42 SUMOylation and activation.Nature communications · 2022Article
- Review
- The Desmosome-Keratin Scaffold Integrates ErbB Family and Mechanical Signaling to Polarize Epidermal Structure and Function.Frontiers in cell and developmental biology · 2022Review
- EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.The Journal of cell biology · 2021Article
- Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony.International journal of molecular sciences · 2021Article
- Label-free quality control and identification of human keratinocyte stem cells by deep learning-based automated cell tracking.Stem cells (Dayton, Ohio) · 2021Article
- Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro.Cells · 2021Article
- Single-keratinocyte transcriptomic analyses identify different clonal types and proliferative potential mediated by FOXM1 in human epidermal stem cells.Nature communications · 2021Article
- Toward Combined Cell and Gene Therapy for Genodermatoses.Cold Spring Harbor perspectives in biology · 2020Review
- Automated collective motion analysis validates human keratinocyte stem cell cultures.Scientific reports · 2019Article
- In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics.Scientific reports · 2019Article
- Orchestrated control of filaggrin-actin scaffolds underpins cornification.Cell death & disease · 2018Article
- Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond.EMBO molecular medicine · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured human epidermal keratinocyte stem cells (holoclones) are crucial for regenerative medicine for burns and genetic disorders. In serial culture, holoclones progressively lose their proliferative capacity to become transient amplifying cells with limited growth (paraclones), a phenomenon termed clonal conversion. Although it negatively impacts the culture lifespan and the success of cell transplantation, little is known on the molecular mechanism underlying clonal conversion. Here, we show that holoclones and paraclones differ in their actin filament organization, with actin bundles distributed radially in holoclones and circumferentially in paraclones. Moreover, actin organization sets the stage for a differing response to epidermal growth factor (EGF), since EGF signalling induces a rapid expansion of colony size in holoclones and a significant reduction in paraclones. Furthermore, inhibition of PI3K or Rac1 in holoclones results in the reorganization of actin filaments in a pattern that is similar to that of paraclones. Importantly, continuous Rac1 inhibition in holoclones results in clonal conversion and reduction of growth potential. Together, our data connect loss of stem cells to EGF-induced colony dynamics governed by Rac1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.