ArticleThe Laryngoscope2013
NTHi induction of Cxcl2 and middle ear mucosal metaplasia in mice.
Article in The Laryngoscope, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Trans-cortical vessels in the mouse temporal bulla bone are a means to recruit myeloid cells in chronic otitis media and limit peripheral leukogram changes.Frontiers in genetics · 2022Article
- The transcriptional landscape of the cultured murine middle ear epithelium in vitro.Biology open · 2021Article
- Subversion of host immune responses by otopathogens during otitis media.Journal of leukocyte biology · 2019Review
- A2ML1 and otitis media: novel variants, differential expression, and relevant pathways.Human mutation · 2019Article
- Nontypeable Haemophilus influenzae lysates increase heterogeneous nuclear ribonucleoprotein secretion and exosome release in human middle-ear epithelial cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Panel 4: Report of the Microbiology Panel.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2017Review
- Panel 3: Genetics and Precision Medicine of Otitis Media.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2017Review
- An in vitro model of murine middle ear epithelium.Disease models & mechanisms · 2016Article
- Dexamethasone modulation of MUC5AC and MUC2 gene expression in a generalized model of middle ear inflammation.The Laryngoscope · 2016Article
- A proteomic characterization of NTHi lysates.International journal of pediatric otorhinolaryngology · 2016Article
- SuperSILAC Quantitative Proteome Profiling of Murine Middle Ear Epithelial Cell Remodeling with NTHi.PloS one · 2016Article
- Nasal Mucociliary Clearance in Adenoid Hypertrophy and Otitis Media with Effusion.Current allergy and asthma reports · 2015Review
- The transcriptome of a complete episode of acute otitis media.BMC genomics · 2015Article
- Role of innate immunity in the pathogenesis of otitis media.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
objectives/hypothesisChronic otitis media (COM) develops after sustained inflammation and is characterized by secretory middle ear epithelial metaplasia and effusion, most frequently mucoid. Nontypeable Haemophilus influenzae (NTHi), the most common acute otitis media (OM) pathogen, is known to activate inflammation and mucin expression in vitro and in animal models of OM. The goals of this study were to examine histopathological and expression profiling epithelial effects of NTHi challenge in murine middle ears. STUDY
designIn vitro and in vivo murine model of OM.
methodsWeekly transtympanic inoculation of Balb/c mice with 300 μg/ml of NTHi lysates versus saline was performed. Histopathologic analysis was carried out at 4 weeks. Expression microarray analysis was performed at 1 and 7 days. Microarray findings were validated in independent animal samples and in a cultured murine middle ear epithelial cell (mMEEC) line.
resultsHistopathologic analyses revealed middle ear mucosal thickening after NTHi exposure. Microarray analyses of inflammatory response genes which changed significantly demonstrated that the chemokine Cxcl2 had the largest fold-change, with significantly increased expression at 1 and 7 days after NTHi injection compared to either saline or no-injection (P <0.01). Validation by real-time qPCR revealed similar significantly increased relative mRNA levels for Cxcl2. NTHi lysates were also found to significantly upregulate the transcription of Cxcl2 in mMEEC in a time- and dose-dependent manner (P <0.05).
conclusionsMiddle ear NTHi challenge in mice leads to chronic epithelial mucosal metaplasia and overexpression of inflammatory mediators, most notably Cxcl2. This finding is parallel to NTHi-mediated pulmonary mucosal metaplasia where Cxcl2 has been identified as an important inflammatory mediator.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.