ArticleCytotechnology2014
Effects of long-term serial cell passaging on cell spreading, migration, and cell-surface ultrastructures of cultured vascular endothelial cells.
Article in Cytotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Quantitative analysis of human umbilical vein endothelial cell morphology and tubulogenesis.Journal of microscopy · 2025Article
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- Targeting Runx1 in Pathological Retinal Angiogenesis: A Potential Therapeutic Approach.Investigative ophthalmology & visual science · 2025Article
- Studying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2024Article
- Global Transcriptome Analysis Reveals Distinct Phases of the Endothelial Response to TNF.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Serial Passaging Affects Stromal Cell Mechanosensitivity on Hyaluronic Acid Hydrogels.Macromolecular bioscience · 2024Article
- Long-term passage impacts human dental pulp stem cell activities and cell response to drug additionPeerJ · 2024Article
- Article
- Quantification of SARS-CoV-2 spike protein expression from mRNA vaccines using isotope dilution mass spectrometry.Vaccine · 2023Article
- Serial passaging affects stromal cell mechanosensitivity on hyaluronic acid hydrogels.bioRxiv : the preprint server for biology · 2023Article
- Atherosclerotic-Derived Endothelial Cell Response Conducted by Titanium Oxide Nanotubes.Materials (Basel, Switzerland) · 2023Article
- 3D printing biocompatible materials with Multi Jet Fusion for bioreactor applications.International journal of bioprinting · 2023Article
- HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes.Biomolecules · 2022Article
- Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue.Marine drugs · 2022Article
- SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells.Scientific reports · 2022Article
- Mechanistic characterization of endothelial sprouting mediated by pro-angiogenic signaling.Microcirculation (New York, N.Y. : 1994) · 2022Article
- Mechanical regulation of signal transduction in angiogenesis.Frontiers in cell and developmental biology · 2022Review
- High metabolic substrate load induces mitochondrial dysfunction in rat skeletal muscle microvascular endothelial cells.Physiological reports · 2021Article
- Vimentin Plays a Crucial Role in Fibroblast Ageing by Regulating Biophysical Properties and Cell Migration.Cells · 2019Article
- Hyperglycaemia cause vascular inflammation through advanced glycation end products/early growth response-1 axis in gestational diabetes mellitus.Molecular and cellular biochemistry · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of serial cell passaging on cell spreading, migration, and cell-surface ultrastructures have been less investigated directly. This study evaluated the effects of long-term serial cell passaging (totally 35 passages) on cultured human umbilical vein endothelial cells which were pre-stored at -80 °C as usual. Percentage- and spread area-based spreading assays, measurements of fluorescently labeled actin filaments, migration assay, and measurements of cell-surface roughness were performed and quantitatively analyzed by confocal microscopy or atomic force microscopy. We found that the abilities of cell spreading and migration first increased at early passages and then decreased after passage 15, in agreement with the changes in average length of actin filaments. Recovery from cold storage and effects of cell passaging were potentially responsible for the increases and decreases of the values, respectively. In contrast, the average roughness of cell surfaces (particularly the nucleus-surrounding region) first dropped at early passages and then rose after passage 15, which might be caused by cold storage- and cell passaging-induced endothelial microparticles. Our data will provide important information for understanding serial cell passaging and implies that for pre-stored adherent cells at -80 °C cell passages 5-10 are optimal for in vitro studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.