ArticleAmerican journal of physiology. Renal physiology2013
Movement of NH₃ through the human urea transporter B: a new gas channel.
Article in American journal of physiology. Renal physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 33 citations in OpenAlex.
- Erythroid anion exchanger-1 (SLC4A1) modulates carbonic anhydrase inhibitor acetazolamide on respiration.Frontiers in physiology · 2026Article
- Ammonia transport mediated by urea transporter A isoforms.Biology open · 2025Article
- Role of channels in thebioRxiv : the preprint server for biology · 2025Article
- Role of channels in the ObioRxiv : the preprint server for biology · 2025Article
- Protein Structures of Urea Transporters.Sub-cellular biochemistry · 2025Review
- Water transport mediated by murine urea transporters: implications for urine concentration mechanisms.Biology open · 2020Article
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Article
- Alternative channels for urea in the inner medulla of the rat kidney.American journal of physiology. Renal physiology · 2015Article
- CrossTalk proposal: Physiological CO2 exchange can depend on membrane channels.The Journal of physiology · 2015Article
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Functional characterization of Na+/H+ exchangers of intracellular compartments using proton-killing selection to express them at the plasma membrane.Journal of visualized experiments : JoVE · 2015Article
- Mathematical modeling of acid-base physiology.Progress in biophysics and molecular biology · 2015Review
- Computing membrane-AQP5-phosphatidylserine binding affinities with hybrid steered molecular dynamics approach.Molecular membrane biology · 2015Article
- Evidence from simultaneous intracellular- and surface-pH transients that carbonic anhydrase II enhances CO2 fluxes across Xenopus oocyte plasma membranes.American journal of physiology. Cell physiology · 2014Article
- Carbon monoxide, hydrogen sulfide, and nitric oxide as signaling molecules in the gastrointestinal tract.Gastroenterology · 2014Review
- Structure of urea transporters.Sub-cellular biochemistry · 2014Review
- Relative CO₂/NH₃ permeabilities of human RhAG, RhBG and RhCG.The Journal of membrane biology · 2013Article
- Energetic and molecular water permeation mechanisms of the human red blood cell urea transporter B.PloS one · 2013Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
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Abstract
Aquaporins and Rh proteins can function as gas (CO₂ and NH₃) channels. The present study explores the urea, H₂O, CO₂, and NH₃ permeability of the human urea transporter B (UT-B) (SLC14A1), expressed in Xenopus oocytes. We monitored urea uptake using [¹⁴C]urea and measured osmotic water permeability (Pf) using video microscopy. To obtain a semiquantitative measure of gas permeability, we used microelectrodes to record the maximum transient change in surface pH (ΔpHS) caused by exposing oocytes to 5% CO₂/33 mM HCO₃⁻ (pHS increase) or 0.5 mM NH₃/NH₄⁺ (pHS decrease). UT-B expression increased oocyte permeability to urea by >20-fold, and Pf by 8-fold vs. H₂O-injected control oocytes. UT-B expression had no effect on the CO₂-induced ΔpHS but doubled the NH₃-induced ΔpHS. Phloretin reduced UT-B-dependent urea uptake (Jurea*) by 45%, Pf* by 50%, and (- ΔpHS*)NH₃ by 70%. p-Chloromercuribenzene sulfonate reduced Jurea* by 25%, Pf* by 30%, and (ΔpHS*)NH₃ by 100%. Molecular dynamics (MD) simulations of membrane-embedded models of UT-B identified the monomeric UT-B pores as the main conduction pathway for both H₂O and NH₃ and characterized the energetics associated with permeation of these species through the channel. Mutating each of two conserved threonines lining the monomeric urea pores reduced H₂O and NH₃ permeability. Our data confirm that UT-B has significant H₂O permeability and for the first time demonstrate significant NH₃ permeability. Thus the UTs become the third family of gas channels. Inhibitor and mutagenesis studies and results of MD simulations suggest that NH₃ and H₂O pass through the three monomeric urea channels in UT-B.
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