ArticleMolecular biology of the cell2013
RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- E- and N-cadherin drive hepatic polarity and lumen elongation via opposing effects on RhoA activity.The Journal of cell biology · 2026Article
- MRCKα represses GEF-H1 mediated RhoA activation to promote ovarian cancer spheroid growth and invasion.bioRxiv : the preprint server for biology · 2026Article
- E- and N-cadherin drive hepatic polarity and lumen elongation via opposing effects on RhoA activity.bioRxiv : the preprint server for biology · 2025Article
- E-Cadherin: A conductor of cellular signaling.Current opinion in cell biology · 2025Review
- Plakophilin 4 controls the spatio-temporal activity of RhoA at adherens junctions to promote cortical actin ring formation and tissue tension.Cellular and molecular life sciences : CMLS · 2024Article
- TGFBR1*6A as a modifier of breast cancer risk and progression: advances and future prospects.NPJ breast cancer · 2022Review
- A Chemomechanical Model for Regulation of Contractility in the Embryonic Brain Tube.Journal Of Elasticity · 2021Article
- Impaired autophagic degradation of lncRNA ARHGAP5-AS1 promotes chemoresistance in gastric cancer.Cell death & disease · 2019Article
- p190RhoGAPs, theCells · 2019Review
- Regulation of Rho GTPase activity at the leading edge of migrating cells by p190RhoGAP.Small GTPases · 2019Review
- Snail-induced claudin-11 prompts collective migration for tumour progression.Nature cell biology · 2019Article
- Signaling by Small GTPases at Cell-Cell Junctions: Protein Interactions Building Control and Networks.Cold Spring Harbor perspectives in biology · 2018Review
- Wild-type p53 enhances endothelial barrier function by mediating RAC1 signalling and RhoA inhibition.Journal of cellular and molecular medicine · 2018Article
- Rho GTPases and actomyosin: Partners in regulating epithelial cell-cell junction structure and function.Experimental cell research · 2017Review
- Loss of miR-203 regulates cell shape and matrix adhesion through ROBO1/Rac/FAK in response to stiffness.The Journal of cell biology · 2016Article
- p120-catenin controls contractility along the vertical axis of epithelial lateral membranes.Journal of cell science · 2016Article
- Rho-associated kinase signalling and the cancer microenvironment: novel biological implications and therapeutic opportunities.Expert reviews in molecular medicine · 2015Review
- Running with neighbors: coordinating cell migration and cell-cell adhesion.Current opinion in cell biology · 2015Review
- Relationship of and cross-talk between physical and biologic properties of the glomerulus.Current opinion in nephrology and hypertension · 2015Review
- Rac1 functions as a reversible tension modulator to stabilize VE-cadherin trans-interaction.The Journal of cell biology · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Breast epithelial cells cultured in three-dimensional (3D) collagen gels undergo ductal morphogenesis when the gel is compliant and they can achieve tensional homeostasis. We previously showed that this process requires down-regulation of Rho in compliant collagen gels, but the mechanism remains undefined. In this study, we find that p190RhoGAP-B, but not p190RhoGAP-A, mediates down-regulation of RhoA activity and ductal morphogenesis in T47D cells cultured in compliant 3D collagen gels. In addition, both RhoA and p190RhoGAP-B colocalize with p120-catenin at sites of cell-cell contact. The association between p190RhoGAP-B and p120-catenin is regulated by matrix compliance such that it increases in compliant vs. rigid collagen gels. Furthermore, knockdown of p120-catenin disrupts ductal morphogenesis, disregulates RhoA activity, and results in loss of p190B at cell-cell contacts. Consistent with these findings, using a RhoA-specific FRET biosensor (RhoA-FLARE.sc), we determined spatial RhoA activity to be significantly decreased at cell-cell contacts versus cell-ECM adhesions, and, of importance, spatial RhoA activity is regulated by p190B. This finding suggests that RhoA exists as an inactive pool at cell-cell contacts and is recruited to cell-ECM contacts within stiff matrices. Overall, these results demonstrate that RhoA is down-regulated at cell-cell contacts through p190RhoGAP-B, which is localized to cell-cell contacts by association with p120-catenin that is regulated by tensional homeostasis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.