ArticleReproductive biology and endocrinology : RB&E2013
Vitrification preserves chromatin integrity, bioenergy potential and oxidative parameters in mouse embryos.
Article in Reproductive biology and endocrinology : RB&E, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- Reactive oxygen species attenuation improves the quality of vitrified-warmed bovine embryos.Animal reproduction · 2025Article
- Transcriptomic Analysis of Vitrified-Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact.International journal of molecular sciences · 2024Article
- Article
- Current and Future Perspectives for Improving Ovarian Tissue Cryopreservation and Transplantation Outcomes for Cancer Patients.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model.Mycotoxin research · 2021Article
- Article
- Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage.PloS one · 2017Article
- Different chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification.Reproductive biology and endocrinology : RB&E · 2015Article
- Prediction of pregnancy viability in bovine in vitro-produced embryos and recipient plasma with Fourier transform infrared spectroscopy.Journal of dairy science · 2014Article
- Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels.Journal of assisted reproduction and genetics · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was to evaluate the effects of vitrification on morpho-functional parameters (blastomere/chromatin integrity and bioenergy/oxidative potential) of mouse preimplantation embryos.
methodsIn vivo produced mouse (4/16-cell, morulae and blastocyst-stage) embryos were randomly divided into vitrification and control groups. For vitrification, embryos were exposed to a 2-step loading of ethylene glycol and propylene glycol, before being placed in a small nylon loop and submerged into liquid nitrogen. After warming, the cryoprotectants were diluted by a 3-step procedure. Embryo morphology, chromatin integrity and energy/oxidative status were compared between groups.
resultsVitrification induced low grade blastomere cytofragmentation (P < 0.05) and low chromatin damage only in embryos at the morula stage (P < 0.001). Mitochondrial (mt) distribution pattern was affected by vitrification only in early embryos (P < 0.001). Mitochondrial activity did not change upon vitrification in morula-stage embryos but it was reduced in blastocyst-stage embryos (P < 0.05). Intracellular ROS levels significantly increased in embryos at the morula and blastocyst stages (P < 0.001). Colocalization of active mitochondria and ROS increased only in vitrified blastocysts.
conclusionsIn conclusion, this study elucidates the developmentally-related and mild effects of vitrification on morphology, nuclear and bioenergy/oxidative parameters of mouse embryos and demonstrates that vitrification is a suitable method for preserving predictive parameters of embryo ability to induce a full-term pregnancy.
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