ArticleOncogenesis2012
Functional consequences of WNT3/Frizzled7-mediated signaling in non-transformed hepatic cells.
Article in Oncogenesis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- FZD7 expression marks mammary tumor-initiating cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Wnt Signaling in Hepatocellular Carcinoma: Biological Mechanisms and Therapeutic Opportunities.Cells · 2024Review
- DNMT3A-mediated DNA methylation and transcription inhibition of FZD5 suppresses lung carcinogenesis.Heliyon · 2024Article
- LINC01023 Promotes the Hepatoblastoma Tumorigenesis via miR-378a-5p/WNT3 Axis.Molecular and cellular biochemistry · 2023Article
- WNT ligands in non-small cell lung cancer: from pathogenesis to clinical practice.Discover oncology · 2023Review
- Prognostic model for hepatocellular carcinoma based on anoikis-related genes: immune landscape analysis and prediction of drug sensitivity.Frontiers in medicine · 2023Article
- FZD5 prevents epithelial-mesenchymal transition in gastric cancer.Cell communication and signaling : CCS · 2021Article
- Downregulation of Wnt3 Suppresses Colorectal Cancer Development Through Inhibiting Cell Proliferation and Migration.Frontiers in pharmacology · 2019Article
- Garlic-derived compoundActa pharmaceutica Sinica. B · 2018Article
- Frizzled7 Promotes Epithelial-to-mesenchymal Transition and Stemness Via Activating Canonical Wnt/β-catenin Pathway in Gastric Cancer.International journal of biological sciences · 2018Article
- Characterizing the activation of the Wnt signaling pathway in hilar cholangiocarcinoma using a tissue microarray approach.European journal of histochemistry : EJH · 2016Article
- Downregulation of human Wnt3 in gastric cancer suppresses cell proliferation and induces apoptosis.OncoTargets and therapy · 2016Article
- Wnt-/-β-catenin pathway signaling in human hepatocellular carcinoma.World journal of hepatology · 2015Article
- Twist in hepatocellular carcinoma: pathophysiology and therapeutics.Hepatology international · 2015Review
- The WNT receptor FZD7 is required for maintenance of the pluripotent state in human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Loss of exon 4 in a human T-cell factor-4 isoform promotes hepatic tumourigenicity.Liver international : official journal of the International Association for the Study of the Liver · 2013Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
We have previously demonstrated that WNT3 and Frizzled7 (FZD7) expression levelswere upregulated in hepatocellular carcinoma (HCC) and that they directly interact to activate the canonical Wnt/β-catenin pathway in HCC cell lines. In this study, we investigated the functional consequences of WNT3 and FZD7 expression levels in non-transformed hepatic cells to address the question of whether WNT3/FZD7-mediated signal transduction could be involved in cellular transformation. After stable transfection of WNT3 and FZD7, the activation of the Wnt/β-catenin pathway was confirmed by western blot, immunostaining and quantitative real-time reverse transcriptase-PCR (qRT-PCR) analysis in two non-transformed hepatocyte-derived cell lines. In vitro characteristics of the malignant phenotype were measured, including cell proliferation, migration, invasion and anchorage-independent growth in soft agar. Stable expression of WNT3 and FZD7 in the two cell lines led to cellular accumulation of β-catenin and expression of downstream target genes activated by this pathway. In the stable WNT3/FZD7-expressing clones, hepatic cell proliferation, migration, invasion as well as soft agar colony formation were enhanced compared with the non-transformed control cells. The epithelial-mesenchymal transition (EMT) factors, Twist, Snail and Vimentin, were increased in cells expressing WNT3 and FZD7. However, the WNT3/FZD7-expressing cells did not form tumors in vivo. We conclude that activation of the WNT3/FZD7 canonical pathway has a role in the early stages of hepatocarcinogenesis by promoting the acquisition of a malignant phenotype with features of EMT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.