ArticleBiotechnology for biofuels2013
Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 81 citations in OpenAlex.
- Development ofBiotechnology reports (Amsterdam, Netherlands) · 2026Article
- Manipulating the nucleolar serine-rich protein Srp40p in Saccharomyces cerevisiae may improve isobutanol production.World journal of microbiology & biotechnology · 2024Article
- Reprogramming inInternational journal of molecular sciences · 2023Article
- Glucose/Xylose Co-FermentingFrontiers in bioengineering and biotechnology · 2022Article
- Tryptophan plays an important role in yeast's tolerance to isobutanol.Biotechnology for biofuels · 2021Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy.Applied microbiology and biotechnology · 2020Article
- Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges.World journal of microbiology & biotechnology · 2020Review
- Knockout of acetoacetate degradation pathway gene3 Biotech · 2019Article
- Phenotype-Independent Isolation of InterspeciesFrontiers in microbiology · 2019Article
- Genotype-by-Environment-by-Environment Interactions in theG3 (Bethesda, Md.) · 2018Article
- Selection of PofFrontiers in microbiology · 2018Article
- Evolutionary engineering improves tolerance for medium-chain alcohols inBiotechnology for biofuels · 2018Article
- The sole introduction of two single-point mutations establishes glycerol utilization inBiotechnology for biofuels · 2017Article
- Evolutionary Engineering in Chemostat Cultures for Improved Maltotriose Fermentation Kinetics inFrontiers in microbiology · 2017Article
- Growth inhibition of S. cerevisiae, B. subtilis, and E. coli by lignocellulosic and fermentation products.Applied microbiology and biotechnology · 2016Article
- Alternative reactions at the interface of glycolysis and citric acid cycle in Saccharomyces cerevisiae.FEMS yeast research · 2016Article
- Replacement of the initial steps of ethanol metabolism in Saccharomyces cerevisiae by ATP-independent acetylating acetaldehyde dehydrogenase.FEMS yeast research · 2016Article
- A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations.Biotechnology for biofuels · 2016Article
- ChiNet uncovers rewired transcription subnetworks in tolerant yeast for advanced biofuels conversion.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundn-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of the molecular basis of butanol stress and tolerance of S. cerevisiae is important for achieving improved tolerance.
resultsBy combining a screening of the haploid S. cerevisiae knock-out library, gene overexpression, and genome analysis of evolutionary engineered n-butanol-tolerant strains, we established that protein degradation plays an essential role in tolerance. Strains deleted in genes involved in the ubiquitin-proteasome system and in vacuolar degradation of damaged proteins showed hypersensitivity to n-butanol. Overexpression of YLR224W, encoding the subunit responsible for the recognition of damaged proteins of an ubiquitin ligase complex, resulted in a strain with a higher n-butanol tolerance. Two independently evolved n-butanol-tolerant strains carried different mutations in both RPN4 and RTG1, which encode transcription factors involved in the expression of proteasome and peroxisomal genes, respectively. Introduction of these mutated alleles in the reference strain increased butanol tolerance, confirming their relevance in the higher tolerance phenotype. The evolved strains, in addition to n-butanol, were also more tolerant to 2-butanol, isobutanol and 1-propanol, indicating a common molecular basis for sensitivity and tolerance to C3 and C4 alcohols.
conclusionsThis study shows that maintenance of protein integrity plays an essential role in butanol tolerance and demonstrates new promising targets to engineer S. cerevisiae for improved tolerance.
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Registered trials
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